Song Huan, He Xiaoyun, Zou Shiying, Zhang Teng, Luo Yunbo, Huang Kunlun, Zhu Zhen, Xu Wentao
College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Tsinghua Donglu, Beijing, 100083, China.
Transgenic Res. 2015 Apr;24(2):295-308. doi: 10.1007/s11248-014-9844-6. Epub 2014 Nov 1.
Bacillus thuringiensis (Bt) transgenic rice line (mfb-MH86) expressing a synthetic cry1Ab gene can be protected against feeding damage from Lepidopteran insects, including Sesamia inferens, Chilo suppressalis, Tryporyza incertulas and Cnaphalocrocis medinalis. Rice flour from mfb-MH86 and its near-isogenic control MH86 was separately formulated into rodent diets at concentrations of 17.5, 35 and 70 % (w/w) for a 90-day feeding test with rats, and all of the diets were nutritionally balanced. In this study, the responses of rats fed diets containing mfb-MH86 were compared to those of rats fed flour from MH86. Overall health, body weight and food consumption were comparable between groups fed diets containing mfb-MH86 and MH86. Blood samples were collected prior to sacrifice and a few significant differences (p < 0.05) were observed in haematological and biochemical parameters between rats fed genetically modified (GM) and non-GM diets. However, the values of these parameters were within the normal ranges of values for rats of this age and sex, thus not considered treatment related. In addition, upon sacrifice a large number of organs were weighed, macroscopic and histopathological examinations were performed with only minor changes to report. In conclusion, these results demonstrated that no toxic effect was observed in the conditions of the experiment, based on the different parameters assessed. GM rice mfb-MH86 is as safe and nutritious as non-GM rice.
表达合成cry1Ab基因的苏云金芽孢杆菌（Bt）转基因水稻品系（mfb-MH86）能够抵御包括大螟、二化螟、三化螟和稻纵卷叶螟在内的鳞翅目昆虫的取食危害。将mfb-MH86及其近等基因对照MH86的米粉分别以17.5%、35%和70%（w/w）的浓度配制成啮齿动物饲料，对大鼠进行为期90天的喂养试验，所有饲料的营养成分均保持平衡。在本研究中，将喂食含mfb-MH86饲料的大鼠的反应与喂食MH86米粉的大鼠的反应进行了比较。喂食含mfb-MH86和MH86饲料的两组大鼠在总体健康状况、体重和食物消耗量方面相当。在处死大鼠前采集了血液样本，在转基因饲料组和非转基因饲料组大鼠的血液学和生化参数方面观察到了一些显著差异（p < 0.05）。然而，这些参数的值在该年龄和性别的大鼠的正常范围内，因此不认为与处理有关。此外，在处死大鼠后，对大量器官进行了称重，并进行了大体和组织病理学检查，仅报告了一些微小变化。总之，基于所评估的不同参数，这些结果表明在实验条件下未观察到毒性作用。转基因水稻mfb-MH86与非转基因水稻一样安全且营养丰富。
