Zeidner Nordin S, Carter Leon G, Monteneiri John A, Petersen Jeannine M, Schriefer Martin, Gage Kenneth L, Hall Greg, Chu May C
Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80522, USA.
J Vet Diagn Invest. 2004 Mar;16(2):150-2. doi: 10.1177/104063870401600210.
An immunohistochemical assay was developed and tested for detection of Francisella tularensis lipopolysaccaride antigen in tissues of captive prairie dogs (Cynomys ludovicianus). Tissues from 59 cases of F. tularensis were examined by this technique, which was corroborated by direct fluorescent antibody assay and direct isolation of the organism. In infected prairie dogs, studies indicated multiple, severe, necroprurulent foci occurring in the liver, lung, spleen, terminal ileum, and mandibular lymph node. Immunohistochemical analysis of the same formalin-fixed tissues indicated the presence of F. tularensis antigen in neutrophils and macrophages of these lesions and occurring extracellularly in areas of necrosis. This report demonstrates that immunohistochemical analysis is a rapid procedure that can be used to determine the pathogenesis of F. tularensis in rodent populations.
开发并测试了一种免疫组织化学检测方法，用于检测圈养草原犬鼠（黑尾草原犬鼠）组织中的土拉弗朗西斯菌脂多糖抗原。采用该技术对59例土拉弗朗西斯菌病例的组织进行了检查，并通过直接荧光抗体检测和该病原体的直接分离得到了证实。在受感染的草原犬鼠中，研究表明，肝脏、肺、脾脏、回肠末端和下颌淋巴结出现多个严重的坏死性脓性病灶。对相同福尔马林固定组织的免疫组织化学分析表明，这些病变的中性粒细胞和巨噬细胞中存在土拉弗朗西斯菌抗原，并且在坏死区域细胞外也有出现。本报告表明，免疫组织化学分析是一种快速的检测方法，可用于确定土拉弗朗西斯菌在啮齿动物群体中的发病机制。
