猪血清中抗猪圆环病毒2型抗体的实验室间检测。

Interlaboratory testing of porcine sera for antibodies to porcine circovirus type 2.

作者信息

McNair Irene, Marshall Michelle, McNeilly Francis, Botner Anette, Ladekjaer-Mikkelsen Anne-Sofie, Vincent Isabelle, Herrmann Brigitte, Sanchez Romeo, Rhodes Carrie

机构信息

Department of Agriculture and Rural Development, Veterinary Sciences Division, Stormont, Belfast BT4 3SD, Northern Ireland.

出版信息

J Vet Diagn Invest. 2004 Mar;16(2):164-6. doi: 10.1177/104063870401600214.

DOI:10.1177/104063870401600214

PMID:15053371

Abstract

A panel of 20 porcine sera was distributed to 5 laboratories across Europe and Canada. Each center was requested to test the sera for the presence of porcine circovirus type 2 antibodies using the routine assays, indirect immunofluorescence assay (IFA) and indirect immunoperoxidase monolayer assay (IPMA), and to determine the titer of each serum. Results from all centers were then compiled and correlated. They demonstrate a wide variation in the titers obtained between laboratories. These differences were dependent on the assay used and the choice of fixative. In general, IPMA gave higher titers than did IFA, and paraformaldehyde gave higher titers than did acetone or ethyl alcohol. This report highlights the need for standardized procedures and biologicals for this virus.

摘要

一组20份猪血清被分发给欧洲和加拿大的5个实验室。要求每个中心使用常规检测方法，即间接免疫荧光法（IFA）和间接免疫过氧化物酶单层分析法（IPMA），检测血清中是否存在猪圆环病毒2型抗体，并确定每份血清的滴度。然后汇总并对比了所有中心的结果。结果表明，各实验室获得的滴度差异很大。这些差异取决于所使用的检测方法和固定剂的选择。一般来说，IPMA获得的滴度高于IFA，多聚甲醛获得的滴度高于丙酮或乙醇。本报告强调了针对该病毒制定标准化程序和生物制品的必要性。