McKhann Heather I, Camilleri Christine, Bérard Aurélie, Bataillon Thomas, David Jacques L, Reboud Xavier, Le Corre Valérie, Caloustian Christophe, Gut Ivo G, Brunel Dominique
Centre National de Génotypage, 2 rue Gaston Crémieux, 91057 Evry Cedex, France.
Plant J. 2004 Apr;38(1):193-202. doi: 10.1111/j.1365-313X.2004.02034.x.
The successful exploitation of natural genetic diversity requires a basic knowledge of the extent of the variation present in a species. To study natural variation in Arabidopsis thaliana, we defined nested core collections maximizing the diversity present among a worldwide set of 265 accessions. The core collections were generated based on DNA sequence data from a limited number of fragments evenly distributed in the genome and were shown to successfully capture the molecular diversity in other loci as well as the morphological diversity. The core collections are available to the scientific community and thus provide an important resource for the study of genetic variation and its functional consequences in Arabidopsis. Moreover, this strategy can be used in other species to provide a rational framework for undertaking diversity surveys, including single nucleotide polymorphism (SNP) discovery and phenotyping, allowing the utilization of genetic variation for the study of complex traits.
成功利用自然遗传多样性需要对一个物种中存在的变异程度有基本的了解。为了研究拟南芥的自然变异，我们定义了嵌套核心种质库，以最大限度地提高全球265份种质资源中的多样性。核心种质库是基于基因组中均匀分布的有限数量片段的DNA序列数据生成的，并被证明能够成功捕获其他位点的分子多样性以及形态多样性。核心种质库可供科学界使用，因此为研究拟南芥的遗传变异及其功能后果提供了重要资源。此外，这种策略可用于其他物种，为开展多样性调查提供合理框架，包括单核苷酸多态性（SNP）发现和表型分析，从而能够利用遗传变异来研究复杂性状。
