School of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA.
Plant J. 2010 Mar;61(6):922-7. doi: 10.1111/j.1365-313X.2009.04030.x.
In 1998 Cereon Genomics LLC, a subsidiary of Monsanto Co., performed a shotgun sequencing of the Arabidopsis thaliana Landsberg erecta genome to a depth of twofold coverage using 'classic' Sanger sequencing. This sequence was assembled and aligned to the Columbia ecotype sequence produced by the Arabidopsis Genome Initiative. The analysis provided tens of thousands of high-confidence predictions of polymorphisms between these two varieties of A. thaliana, and the predicted polymorphisms and Landsberg erecta sequence were subsequently made available to the not-for-profit research community by Monsanto. These data have been used for a wide variety of published studies, including map-based gene identification from forward genetic screens, studies of recombination and organelle genetics, and gene expression studies. The combination of resequencing approaches with next-generation sequencing technology has led to an increasing number of similar studies of genome-wide genetic diversity in A. thaliana, including the 1001 genomes project (http://1001genomes.org). Similar approaches are becoming possible in any number of crop species as DNA sequencing costs plummet and throughput rapidly increases, promising to lay the groundwork for revolutionizing our understanding of the relationship between genotype and phenotype in plants.
1998 年，孟山都公司的子公司 Cereon Genomics LLC 采用“经典”桑格测序法对拟南芥 LANDBERG erecta 基因组进行了双深度测序。该序列被组装并与由拟南芥基因组计划产生的哥伦比亚生态型序列进行了比对。该分析为数以万计的这两种拟南芥品种之间的多态性提供了高度可信的预测，随后孟山都公司将预测的多态性和 LANDBERG erecta 序列提供给非营利性研究社区。这些数据已被广泛用于各种已发表的研究，包括基于图谱的正向遗传学筛选中的基因鉴定、重组和细胞器遗传学研究以及基因表达研究。重测序方法与下一代测序技术的结合，导致对拟南芥全基因组遗传多样性的越来越多的类似研究，包括 1001 个基因组项目（http://1001genomes.org）。随着 DNA 测序成本的大幅下降和通量的迅速提高，任何数量的作物品种都有可能采用类似的方法，有望为我们理解植物基因型和表型之间的关系带来革命性的变化奠定基础。
