Cui W, Fan Y, Yang M, Zhang Z
Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
Clin Lab Haematol. 2004 Apr;26(2):95-100. doi: 10.1111/j.1365-2257.2004.00586.x.
Lymphocyte analysis has not been accepted as an indicator for the clinical diagnosis of paroxysmal nocturnal hemoglobinuria (PNH), because of diverse CD59 expression, even in the healthy individuals. We studied the expression of CD59 on lymphocytes and lymphocyte subsets in healthy individuals and PNH patients by flow cytometry. In 50 healthy individuals, granulocytes generally showed a single-cell population that was strongly positive for CD59. Lymphocytes showed, in addition to the population of positive cells, a small population of weakly positive cells, while CD3+ T-cells showed a large population of weakly positive cells. Natural killer (NK)-cells showed a profile similar to CD3+ T-cells. CD4+ T-cells showed a larger population of strongly positive cells, as compared with CD3+ T-cells, while CD8+ T-cells showed a smaller population of strongly positive cells. CD20+ B-cells showed a similar profile to granulocytes. CD59 expression studies in all our PNH patients showed that B-cells expressed CD59 at levels similar to granulocytes, which were lower than those of T-cells and NK-cells. Therefore, the lymphocyte population with low level CD59 expression in healthy controls corresponds to T-cells (especially CD8+ T-cells) and NK-cells. CD59 expression on B-cells could thus be used as a new marker for the diagnosis of PNH.
淋巴细胞分析尚未被接受作为阵发性夜间血红蛋白尿（PNH）临床诊断的指标，因为即使在健康个体中，CD59表达也存在差异。我们通过流式细胞术研究了健康个体和PNH患者淋巴细胞及淋巴细胞亚群上CD59的表达。在50名健康个体中，粒细胞通常显示为CD59强阳性的单细胞群体。淋巴细胞除了有阳性细胞群体外，还有一小部分弱阳性细胞，而CD3 + T细胞有大量弱阳性细胞。自然杀伤（NK）细胞表现出与CD3 + T细胞相似的特征。与CD3 + T细胞相比，CD4 + T细胞有更大比例的强阳性细胞，而CD8 + T细胞有较小比例的强阳性细胞。CD20 + B细胞表现出与粒细胞相似的特征。我们所有PNH患者的CD59表达研究表明，B细胞表达的CD59水平与粒细胞相似，低于T细胞和NK细胞。因此，健康对照中CD59低表达的淋巴细胞群体对应于T细胞（尤其是CD8 + T细胞）和NK细胞。B细胞上的CD59表达因此可作为PNH诊断的新标志物。
