Nagakura S, Nakakuma H, Horikawa K, Hidaka M, Kagimoto T, Kawakita M, Tomita M, Takatsuki K
Second Department of Internal Medicine, Kumamoto University School of Medicine, Japan.
Am J Hematol. 1993 May;43(1):14-8. doi: 10.1002/ajh.2830430105.
The expression of phosphatidylinositol (PI)-anchored complement-regulatory membrane proteins on circulating blood cells has been well clarified; however, the PI proteins on lymphocyte subsets have not been fully analyzed yet. We examined the expression of decay-accelerating factor (DAF) and CD59 on the T lymphocytes (CD2+, CD3+, CD4+, and CD8+) and CD20+ B lymphocytes in ten healthy volunteers and 12 paroxysmal nocturnal hemoglobinuria (PNH) patients by cytofluorometry. In healthy controls, each subset of lymphocytes showed a small population of cells weakly positive and a large population of cells strongly positive for DAF and CD59, while erythrocytes showed a single population of cells positive for the PI proteins. The two-population expression of DAF was most distinctive in CD8+ T cells among the subsets. In PNH, each subset of lymphocytes showed a moderately higher population of cells weakly positive and a smaller population of cells strongly positive for the membrane proteins compared with those in the healthy controls. Moreover, in some PNH cases, a negative population for the proteins was found in all subsets. Thus the analysis of PI-anchored proteins on lymphocytes subsets (especially CD8+ T cells) was considered to be of diagnostic value in PNH patients who receive blood transfusion after hemolytic attack of affected erythrocytes. Furthermore, the two-population expression of PI proteins in normal lymphocytes suggests that membrane PI protein would be a new subset marker of lymphocytes.
循环血细胞上磷脂酰肌醇（PI）锚定的补体调节膜蛋白的表达已得到充分阐明；然而，淋巴细胞亚群上的PI蛋白尚未得到充分分析。我们通过细胞荧光测定法检测了10名健康志愿者和12名阵发性夜间血红蛋白尿（PNH）患者的T淋巴细胞（CD2 +、CD3 +、CD4 +和CD8 +）及CD20 + B淋巴细胞上衰变加速因子（DAF）和CD59的表达。在健康对照中，淋巴细胞的每个亚群均显示一小部分细胞DAF和CD59弱阳性，而大部分细胞强阳性，而红细胞则显示单一群体的细胞PI蛋白阳性。在各亚群中，DAF的双群体表达在CD8 + T细胞中最为明显。在PNH患者中，与健康对照相比，淋巴细胞的每个亚群均显示膜蛋白弱阳性的细胞群体略多，而强阳性的细胞群体略少。此外，在一些PNH病例中，所有亚群均发现了蛋白阴性群体。因此，淋巴细胞亚群（尤其是CD8 + T细胞）上PI锚定蛋白的分析被认为对受影响红细胞溶血发作后接受输血的PNH患者具有诊断价值。此外，正常淋巴细胞中PI蛋白的双群体表达表明膜PI蛋白可能是淋巴细胞的一种新的亚群标志物。
