Nardini Martina, Ori Michela, Vigetti Davide, Gornati Rosalba, Nardi Irma, Perris Roberto
Laboratori di Biologia Cellulare e dello Sviluppo, Dipartimento di Fisiologia e Biochimica, Università di Pisa, Via Carducci 13, Ghezzano, Pisa 56010, Italy.
Gene Expr Patterns. 2004 May;4(3):303-8. doi: 10.1016/j.modgep.2003.10.006.
Here reported is the developmental gene expression pattern of the three known vertebrate hyaluronan synthases (XHas1, XHas2 and XHas3) and a comparative analysis of their mRNAs spatio-temporal distribution during Xenopus laevis development. We found that while XHas2 shows a steady-state expression from gastrula to late tailbud stage, XHas1 is mainly present in the early phases of development while XHas3 is predominantly transcribed in tailbud embryos. XHas1, XHas2 and XHas3 show distinct tissue expression patterns. In particular, XHas1 is localized in ectodermal derivatives and in cranial neural crest cells, whereas XHas2 is mainly found in mesoderm-derived structures and in trunk neural crest cells. Moreover, the expression pattern of XHas2 overlaps that of MyoD in cells committed to a muscle fate. Unlike the other hyaluronan synthases, XHas3 mRNA distribution is very restricted. In particular, XHas3 is expressed in the otic vesicles and closely follows the inner ear development. In conclusion, XHas1, XHas2 and XHas3 mRNAs have distinct and never overlapping spatial expression domains, which would suggest that these three enzymes may play different roles during embryogenesis.
本文报道了三种已知脊椎动物透明质酸合酶（XHas1、XHas2和XHas3）的发育基因表达模式，并对它们在非洲爪蟾发育过程中的mRNA时空分布进行了比较分析。我们发现，虽然XHas2从原肠胚期到尾芽后期呈现稳态表达，但XHas1主要存在于发育的早期阶段，而XHas3主要在尾芽胚胎中转录。XHas1、XHas2和XHas3表现出不同的组织表达模式。特别是，XHas1定位于外胚层衍生物和颅神经嵴细胞中，而XHas2主要存在于中胚层来源的结构和躯干神经嵴细胞中。此外，XHas2的表达模式与决定肌肉命运的细胞中MyoD的表达模式重叠。与其他透明质酸合酶不同，XHas3 mRNA的分布非常局限。特别是，XHas3在耳囊中表达，并紧密跟随内耳发育。总之，XHas1、XHas2和XHas3的mRNA具有不同且从不重叠的空间表达域，这表明这三种酶在胚胎发生过程中可能发挥不同的作用。
