Moya Fernando, Valdeolmillos Miguel
Instituto de Neurociencias-CSIC, Universidad Miguel Hernández, Campus de San Juan, Apartado 18, 03550 San Juan de Alicante, Spain.
Cereb Cortex. 2004 Jun;14(6):610-8. doi: 10.1093/cercor/bhh022. Epub 2004 Mar 28.
Cortical interneurons originate from the ganglionic eminences and reach their final position in the cortex via tangential migratory routes. The mechanisms of this migration are poorly understood. Here we have performed confocal time-lapse analysis of cell movement in the intermediate zone of embryonic mouse cortical slices in order to directly visualize their mode of migration. Tangentially migrating neurons moved by nucleokinesis, characterized by active phases of discontinuous advances of the nucleus followed by periods of quiescence. Dissociated cells from the ganglionic eminences also showed nucleokinesis associated with an increase of intracellular calcium, Ca(2+) Calcium elevation was greatest in the proximal region of the leading process, a zone with a wide distribution of gamma-tubulin. General increases in Ca(2+) elicited by microperfussion with neurotransmitters did not elicit nucleokinesis. These results show that tangential migration uses nucleokinesis, a cell-intrinsic process in which calcium signalling is local, directional and highly regulated.
皮质中间神经元起源于神经节隆起，并通过切向迁移途径到达皮质中的最终位置。这种迁移的机制尚不清楚。在这里，我们对胚胎小鼠皮质切片中间区的细胞运动进行了共聚焦延时分析，以便直接观察它们的迁移模式。切向迁移的神经元通过核运动进行移动，其特征是细胞核间歇性地活跃前进，随后是静止期。来自神经节隆起的解离细胞也显示出与细胞内钙[Ca(2+)]i增加相关的核运动。在领先突起的近端区域，钙升高最为明显，该区域有广泛分布的γ-微管蛋白。用神经递质进行微量灌注引起的[Ca(2+)]i普遍升高并未引发核运动。这些结果表明，切向迁移利用核运动，这是一种细胞内在过程，其中钙信号是局部的、定向的且受到高度调节。
