Moon Se-Kwon, Wee Young-Jung, Yun Jong-Sun, Ryu Hwa-Won
School of Biological Sciences and Technology, Institute of Bioindustrial Technology, Chonnam National University, Gwangju 500-757, Korea.
Appl Biochem Biotechnol. 2004 Spring;113-116:843-55. doi: 10.1385/abab:115:1-3:0843.
The fungal production of fumaric acid using rice bran and subsequent bacterial conversion of succinic acid using fungal culture broth were investigated. Since the rice bran contains abundant proteins, amino acids, vitamins, and minerals, it is suitable material that fungi use as a nitrogen source. The effective concentration of rice bran to produce fumaric acid was 5 g/L. A large amount of rice bran caused excessive fungal growth rather than enhance fumaric acid production. In addition, we could produce fumaric acid without the addition of zinc and iron. Fungal culture broth containing approx 25 g/L of fumaric acid was directly employed for succinic acid conversion. The amount of glycerol and yeast extract required for succinic acid conversion was reduced to 70 and 30%, respectively, compared with the amounts cited in previous studies.
研究了利用米糠进行真菌生产富马酸以及随后利用真菌培养液进行细菌将富马酸转化为琥珀酸的过程。由于米糠含有丰富的蛋白质、氨基酸、维生素和矿物质，它是真菌用作氮源的合适材料。用于生产富马酸的米糠有效浓度为5 g/L。大量的米糠会导致真菌过度生长，而不是提高富马酸的产量。此外，我们可以在不添加锌和铁的情况下生产富马酸。含有约25 g/L富马酸的真菌培养液直接用于琥珀酸转化。与先前研究中引用的量相比，琥珀酸转化所需的甘油和酵母提取物的量分别减少到70%和30%。
