Wang X, Gong C S, Tsao G T
Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907, USA.
Appl Biochem Biotechnol. 1998 Spring;70-72:919-28. doi: 10.1007/BF02920202.
Succinic acid was produced efficiently from fumaric acid by a recombinant E. coli strain DH5 alpha/pGC1002 containing multicopy fumarate reductase genes. The effects of initial fumaric acid and glucose concentration on the production of succinic acid were investigated. Succinic acid reached 41 to over 60 g/L in 48.5 h starting with 50 to 64 g/L fumaric acid. Significant substrate inhibition was observed at initial fumaric acid concentration of 90 g/L. L-Malic acid became the major fermentation product under these conditions. Provision of glucose (5-30 g/L) to the fermentation medium stimulated the initial succinic acid production rate over two folds.
通过含有多拷贝富马酸还原酶基因的重组大肠杆菌菌株DH5α/pGC1002，可从富马酸高效生产琥珀酸。研究了初始富马酸和葡萄糖浓度对琥珀酸生产的影响。以50至64 g/L富马酸起始，在48.5小时内琥珀酸产量达到41至60 g/L以上。在初始富马酸浓度为90 g/L时观察到显著的底物抑制。在此条件下，L-苹果酸成为主要发酵产物。向发酵培养基中添加葡萄糖（5 - 30 g/L）可使初始琥珀酸生产速率提高两倍以上。
