Dien Bruce S, Nagle Nick, Hicks Kevin B, Singh Vijay, Moreau Robert A, Tucker Melvin P, Nichols Nancy N, Johnston David B, Cotta Michael A, Nguyen Quang, Bothast Rodney J
National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, USA.
Appl Biochem Biotechnol. 2004 Spring;113-116:937-49.
Approximately 9% of the 9.7 billion bushels of corn harvested in the United States was used for fuel ethanol production in 2002, half of which was prepared for fermentation by dry grinding. The University of Illinois has developed a modified dry grind process that allows recovery of the fiber fractions prior to fermentation. We report here on conversion of this fiber (Quick Fiber [QF]) to ethanol. QF was analyzed and found to contain 32%wt glucans and 65%wt total carbohydrates. QF was pretreated with dilute acid and converted into ethanol using either ethanologenic Escherichia coli strain FBR5 or Saccharomyces cerevisiae. For the bacterial fermentation the liquid fraction was fermented, and for the yeast fermentation both liquid and solids were fermented. For the bacterial fermentation, the final ethanol concentration was 30 g/L, a yield of 0.44 g ethanol/g of sugar(s) initially present in the hydrolysate, which is 85% of the theoretical yield. The ethanol yield with yeast was 0.096 gal/bu of processed corn assuming a QF yield of 3.04 lb/bu. The residuals from the fermentations were also evaluated as a source of corn fiber oil, which has value as a nutraceutical. Corn fiber oil yields were 8.28%wt for solids recovered following pretreatment.
2002年,美国收获的97亿蒲式耳玉米中约有9%用于燃料乙醇生产,其中一半是通过干磨法准备用于发酵的。伊利诺伊大学开发了一种改良的干磨工艺,该工艺能够在发酵前回收纤维部分。我们在此报告这种纤维(快速纤维[QF])转化为乙醇的情况。对QF进行分析后发现,其含有32%(重量)的葡聚糖和65%(重量)的总碳水化合物。将QF用稀酸预处理,然后使用产乙醇的大肠杆菌菌株FBR5或酿酒酵母转化为乙醇。对于细菌发酵,发酵液体部分;对于酵母发酵,则发酵液体和固体部分。对于细菌发酵,最终乙醇浓度为30 g/L,产量为每克最初存在于水解产物中的糖产生0.44 g乙醇,这是理论产量的85%。假设QF产量为3.04磅/蒲式耳,酵母发酵的乙醇产量为0.096加仑/蒲式耳加工玉米。发酵产生的残渣也被评估为玉米纤维油的来源,玉米纤维油作为一种营养保健品具有价值。预处理后回收的固体中玉米纤维油产量为8.28%(重量)。