Wong Brian J f, Zhao Yonghua, Yamaguchi Mark, Nassif Nader, Chen Zhongping, De Boer Johannes F
Beckman Laser Institute and Medical Clinic, University of California, Irvine, CA 92612, USA.
Otolaryngol Head Neck Surg. 2004 Mar;130(3):334-8. doi: 10.1016/j.otohns.2003.11.007.
The purpose of this study was to use high-speed optical coherence tomography (OCT) to obtain cross-sectional images of the rat cochlea.
The cochleae of Sprague- Dawley rats were imaged within 2 to 4 hours after death. Specimens were prepared by removing the bulla to expose the mesotympanum; some images were obtained in intact temporal bones removed from the cranium. The central element of an OCT imaging device is a Michelson interferometer combined with a low-coherence light source. This study used 2 OCT systems with different light sources: 1) a semiconductor optical amplifier operating and 2) a superluminescent diode with peak emissions wavelengths of 1.3 microm and 827 nm, respectively. Images (1.87 x 2.00 mm or 1.87 x 1.00 mm, 10 x 10 microm pixel resolution) were acquired at a frame rate of 1 Hz. Cross-sectional images at 100-microm increments were obtained from a medial-to-lateral direction.
The scala vestibuli, scala media, scala tympani, modiolus, spiral ligament, and several turns of the cochlea were identified.
These images reflect the ability of OCT to provide images of the internal cochlea structure with micron scale resolution and at near-real time frame rates.
本研究旨在使用高速光学相干断层扫描（OCT）获取大鼠耳蜗的横截面图像。
在Sprague-Dawley大鼠死亡后2至4小时内对其耳蜗进行成像。通过去除颞骨泡以暴露中鼓室来制备标本；一些图像是在从颅骨上取下的完整颞骨中获得的。OCT成像设备的核心元件是与低相干光源相结合的迈克尔逊干涉仪。本研究使用了2种具有不同光源的OCT系统：1）一个工作的半导体光放大器和2）一个峰值发射波长分别为1.3微米和827纳米的超发光二极管。以1赫兹的帧率采集图像（1.87×2.00毫米或1.87×1.00毫米，10×10微米像素分辨率）。从内侧到外侧方向以100微米的增量获取横截面图像。
识别出了前庭阶、中阶、鼓阶、蜗轴、螺旋韧带以及耳蜗的几圈。
这些图像反映了OCT以微米级分辨率和近实时帧率提供耳蜗内部结构图像的能力。
