Sepehr Ali, Djalilian Hamid R, Chang Janice E, Chen Zhongping, Wong Brian J F
Beckman Laser Institute and Medical Clinic, University of California-Irvine, USA.
Laryngoscope. 2008 Aug;118(8):1449-51. doi: 10.1097/MLG.0b013e318173dd6c.
OBJECTIVES/HYPOTHESIS: To demonstrate the feasibility of optical coherence tomography in microstructural imaging of the porcine cochlea.
Ex vivo, porcine model.
Optical coherence tomographic images of the porcine cochlea were obtained by thinning the bone from the basal turn of the cochlea leaving the endosteum intact. The images were compared with the corresponding histological sections.
In the areas of thinned bone, images were obtained of the stria vascularis, Reissner's membrane, basilar membrane, tectorial membrane, scala media, scala tympani, and scala vestibuli. The bone was too thick for adequate light penetration in the areas where it was not thinned. Good histological correlation was obtained.
Cochlear and vestibular microanatomic structures of the pig cochlea were clearly identified with histological confirmation, suggesting the potential application of this noninvasive imaging modality for in vivo imaging of the human cochlea.
目的/假设：证明光学相干断层扫描技术在猪耳蜗微观结构成像中的可行性。
体外猪模型。
通过从耳蜗基底转处减薄骨质，同时保持骨内膜完整，获取猪耳蜗的光学相干断层扫描图像。将这些图像与相应的组织学切片进行比较。
在骨质减薄的区域，获得了血管纹、Reissner膜、基底膜、盖膜、中阶、鼓阶和前庭阶的图像。在未减薄的区域，骨质过厚，光线无法充分穿透。获得了良好的组织学相关性。
通过组织学证实，清晰识别了猪耳蜗的耳蜗和前庭微解剖结构，表明这种非侵入性成像方式在人体耳蜗体内成像方面具有潜在应用价值。
