Van Haaster L H, De Jong F H, Docter R, De Rooij D G
Department of Cell Biology, Medical School, Utrecht University, The Netherlands.
Endocrinology. 1992 Sep;131(3):1574-6. doi: 10.1210/endo.131.3.1505485.
In this study we show that 6-propyl-2-thiouracil (PTU) treatment of Wistar rats from birth up to day 26 p.p. retards the morphological differentiation of Sertoli cells, and prolongs the proliferation of these cells up to day 30. Sertoli cell numbers per testis, determined at day 36, were increased by 84% compared to controls. PTU treatment increased serum thyroid-stimulating hormone (TSH) levels and reduced serum levels of thyroxine (T4) from 5 days onwards, indicative of severe hypothyroidism. Follicle-stimulating hormone (FSH) levels were reduced from day 5 to 9, normal at day 12 and 16, and reduced again from day 20 to 36. Inhibin levels were decreased from day 9 to 20 and increased at 36 days of age. The increase in the number of Sertoli cells per testis in PTU treated rats, as has been reported in the present study, is likely to be responsible for the increased testis size observed by other groups (1) in these animals, when adult.
在本研究中，我们发现，从出生到出生后第26天，用6-丙基-2-硫氧嘧啶（PTU）处理Wistar大鼠，会延缓支持细胞的形态分化，并将这些细胞的增殖延长至第30天。在第36天测定时，每个睾丸的支持细胞数量与对照组相比增加了84%。从第5天起，PTU处理会增加血清促甲状腺激素（TSH）水平，并降低血清甲状腺素（T4）水平，这表明存在严重的甲状腺功能减退。促卵泡激素（FSH）水平在第5天至第9天降低，在第12天和第16天正常，在第20天至第36天再次降低。抑制素水平在第9天至第20天降低，在36日龄时升高。如本研究报道，PTU处理的大鼠每个睾丸中支持细胞数量的增加，可能是其他研究小组在这些动物成年时观察到睾丸体积增大的原因。
