Morales Marcia, Velázquez Elia, Jan Janet, Revah Sergio, González Uriel, Razo-Flores Elías
Programa de Medio Ambiente y Seguridad, Instituto Mexicano del Petróleo, Eje Central Lazzaro Cárdenas 152, C.P 07730, Mexico DF.
Biotechnol Lett. 2004 Feb;26(4):269-75. doi: 10.1023/b:bile.0000015424.04776.55.
Microbial consortia obtained from soil samples of gasoline-polluted sites were individually enriched with pentane, hexane, isooctane and toluene. Cometabolism with methyl tert-butyl ether, (MTBE), gave maximum degradation rates of 49, 12, 32 and 0 mg g(-1)protein h(-1), respectively. MTBE was fully degraded even when pentane was completely depleted with a cometabolic coefficient of 1 mgMTBE mg(-1)pentane. The analysis of 16S rDNA from isolated microorganisms in the pentane-adapted consortia showed that microorganisms could be assigned to Pseudomonas. This is the first work reporting the cometabolic mineralization of MTBE by consortium of this genus.
从汽油污染场地的土壤样本中获得的微生物群落分别用戊烷、己烷、异辛烷和甲苯进行富集培养。与甲基叔丁基醚（MTBE）的共代谢作用分别产生了49、12、32和0 mg g(-1)蛋白质 h(-1)的最大降解率。即使戊烷完全耗尽，MTBE仍能完全降解，共代谢系数为1 mgMTBE mg(-1)戊烷。对戊烷适应菌群中分离出的微生物的16S rDNA分析表明，这些微生物可归为假单胞菌属。这是首次报道该属菌群对MTBE进行共代谢矿化的研究。
