Noël Marilyne, Lozano Pedro, Vaultier Michel, Iborra José L
Departamento de Bioquímica y Biología Molecular B e Inmunología, Facultad de Química, Universidad de Murcia, P.O. Box 4021, E-30100 Murcia, España.
Biotechnol Lett. 2004 Feb;26(4):301-6. doi: 10.1023/b:bile.0000015429.28402.50.
The ionic liquid, l-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide ([Bmim] [NTf2]), was used as a reaction medium for the kinetic resolution of rac-2-pentanol catalyzed by free Candida antarctica lipase B, using vinyl propionate at 2% (v/v) water content. The synthetic activity of lipase in [Bmim] [NTf2] was up 2.5-times greater than in hexane, and showed high enantioselectivity (ee > 99.99%). The optimal temperature and pH were 60 degrees C and 7, respectively. A decrease in water activity (aw) produced a decay in synthetic activity, and an exponential increase in selectivity.
离子液体1-丁基-3-甲基咪唑双[（三氟甲基）磺酰基]酰胺（[Bmim][NTf2]）被用作反应介质，用于在2%（v/v）水含量下，由游离的南极假丝酵母脂肪酶B催化外消旋2-戊醇的动力学拆分，使用丙酸乙烯酯。脂肪酶在[Bmim][NTf2]中的合成活性比在己烷中高2.5倍，并表现出高对映选择性（ee>99.99%）。最佳温度和pH分别为60℃和7。水活度（aw）的降低导致合成活性下降，选择性呈指数增加。
