脯氨酸类似物[2,3-³H]氮杂环丁烷-2-羧酸的合成。在拟南芥和大肠杆菌中的摄取与掺入。

Synthesis of the proline analogue [2,3-3H]azetidine-2-carboxylic acid. Uptake and incorporation in Arabidopsis thaliana and Escherichia coli.

作者信息

Verbruggen N, van Montagu M, Messens E

机构信息

Laboratorium voor Genetica, Universiteit Gent, Belgium.

出版信息

FEBS Lett. 1992 Aug 24;308(3):261-3. doi: 10.1016/0014-5793(92)81288-w.

DOI:10.1016/0014-5793(92)81288-w

PMID:1505664

Abstract

Azetidine-2-carboxylic acid, the 4-membered ring noranalogue of proline, is regularly used in the study of proline metabolism as well as the study of protein conformation. We prepared D,L-[2,3-3H]azetidine-2-carboxylic acid with an optimized 10% yield from commercially available 4-amino-[2,3-3H]butyric acid. Purification was performed by fast-protein liquid chromatography. The biological activity was checked in both Arabidopsis thaliana and Escherichia coli. The obtained specific activity of 10 mCi/mmol was sufficient for most uptake and incorporation studies.

摘要

氮杂环丁烷-2-羧酸是脯氨酸的四元环去甲类似物，常用于脯氨酸代谢研究以及蛋白质构象研究。我们以市售的4-氨基-[2,3-³H]丁酸为原料，通过优化反应，以10%的产率制备了D,L-[2,3-³H]氮杂环丁烷-2-羧酸。纯化通过快速蛋白质液相色谱法进行。在拟南芥和大肠杆菌中均检测了其生物活性。所获得的10 mCi/mmol的比活度足以满足大多数摄取和掺入研究的需要。