纳豆芽孢杆菌OK2生物素营养缺陷型菌株中一个不完整生物素操纵子的遗传分析。

Genetic analysis of an incomplete bio operon in a biotin auxotrophic strain of Bacillus subtilis natto OK2.

作者信息

Sasaki Mayumi, Kawamura Fujio, Kurusu Yasurou

机构信息

Laboratory of Molecular Microbiology, College of Agriculture, Ibaraki University, Inashiki, Japan.

出版信息

Biosci Biotechnol Biochem. 2004 Mar;68(3):739-42. doi: 10.1271/bbb.68.739.

DOI:10.1271/bbb.68.739

PMID:15056910

Abstract

We describe the genetic analysis of the bio operon of the biotin auxotrophic Bacillus subtilis natto OK2 strain. The OK2 strain would only cross-feed with the Escherichia coli bioB mutant and also grew well in medium containing dethiobiotin. Sequencing analysis revealed two significant genetic alterations in the bioW and bioF genes within the bio operon of the OK2 strain. Complementation analysis with B. subtilis 168 bio mutants demonstrated that only the bioB gene could complement, but other bio operon genes could not. A bio(+) transformant, isolated from an OK2 strain, has biotin autotrophy.

摘要

我们描述了生物素营养缺陷型纳豆芽孢杆菌OK2菌株生物操纵子的遗传分析。OK2菌株仅能与大肠杆菌bioB突变体进行交叉喂养，并且在含有脱硫生物素的培养基中生长良好。测序分析揭示了OK2菌株生物操纵子内bioW和bioF基因中的两个显著遗传改变。用枯草芽孢杆菌168生物突变体进行的互补分析表明，只有bioB基因能够互补，而其他生物操纵子基因则不能。从OK2菌株分离出的一个bio(+)转化体具有生物素自养能力。