大肠杆菌生物素基因簇中启动子和操纵基因位点的定位。
Location of promoter and operator sites in the biotin gene cluster of Escherichia coli.
作者信息
Cleary P P, Campbell A, Chang R
出版信息
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2219-23. doi: 10.1073/pnas.69.8.2219.
Abstract
Biotin independence in E. coli requires five closely linked genes, bioA, bioB, bioF, bioC, and bioD. The residual gene activity of deletion mutants has been studied by complementation and enzyme assays. Deletion of the left end of the bioA gene does not impair expression of the remaining genes, but deletions from the left extending into bioB abolish all gene expression. Nonsense mutations in bioB reduce expression of bioC, bioF, and bioD. Therefore, the four genes, bioB, bioF, bioC, and bioD, are transcribed as a unit from left to right, from a promotor located between bioA and bioB. Expression of the bio genes is repressible by added biotin. Deletions removing the left end of bioA do not affect repressibility of bioD. Therefore the operator, as well as the promoter, lie to the right of bioA. One deletion that removes bioA, bioB, and bioF renders the bioD gene constitutive, presumably by fusion to an unknown operon. Therefore, the operator lies to the left of bioC.
摘要
大肠杆菌中生物素自主性需要五个紧密连锁的基因，即bioA、bioB、bioF、bioC和bioD。已通过互补和酶分析研究了缺失突变体的残余基因活性。bioA基因左端的缺失不会损害其余基因的表达，但从左端延伸至bioB的缺失会消除所有基因的表达。bioB中的无义突变会降低bioC、bioF和bioD的表达。因此，bioB、bioF、bioC和bioD这四个基因从位于bioA和bioB之间的启动子开始，作为一个单元从左向右转录。生物素基因的表达可被添加的生物素抑制。去除bioA左端的缺失不会影响bioD的可抑制性。因此，操纵基因以及启动子位于bioA的右侧。一个去除bioA、bioB和bioF的缺失使bioD基因组成型表达，推测是通过与一个未知操纵子融合。因此，操纵基因位于bioC的左侧。
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本文引用的文献
1
An early intermediate in the biosynthesis of biotin: Incorporation studies with [1,7-C(2)]pimelic acid.生物素生物合成中的早期中间体：[1,7-C(2)]戊二酸的掺入研究。
Biochem J. 1966 Dec;101(3):601-6. doi: 10.1042/bj1010601.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
THE BIOSYNTHESIS OF BIOTIN IN MICROORGANISMS. I. THE PHYSIOLOGY OF BIOTIN SYNTHESIS IN ESCHERICHIA COLI.微生物中生物素的生物合成。I. 大肠杆菌中生物素合成的生理学
Biochim Biophys Acta. 1965 Apr 12;100:28-35. doi: 10.1016/0304-4165(65)90423-x.
4
REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. 3. MAP ORDER OF THE STRUCTURAL GENES AND OPERATOR GENES.异亮氨酸和缬氨酸生物合成中的调控机制。3. 结构基因和操纵基因的图谱顺序。
J Bacteriol. 1965 Mar;89(3):661-4. doi: 10.1128/jb.89.3.661-664.1965.
5
[THE PROMOTOR, A GENETIC ELEMENT NECESSARY TO THE EXPRESSION OF AN OPERON].[启动子，操纵子表达所必需的遗传元件]
C R Hebd Seances Acad Sci. 1964 Mar 16;258:3125-8.
6
CO-LINEARITY OF THE GENE WITH THE POLYPEPTIDE CHAIN.基因与多肽链的共线性
Nature. 1964 Jan 4;201:13-7. doi: 10.1038/201013a0.
7
The regulatory mechanism of enzyme synthesis in the tryptophan biosynthetic pathway of Escherichia coli K-12.大肠杆菌K-12色氨酸生物合成途径中酶合成的调控机制。
Biochem Biophys Res Commun. 1962 Oct 17;9:204-7. doi: 10.1016/0006-291x(62)90058-x.
8
Sensitive mutants of bacteriophage lambda.噬菌体λ的敏感突变体
Virology. 1961 May;14:22-32. doi: 10.1016/0042-6822(61)90128-3.
9
A strong suppressor specific for UGA.一种对UGA具有特异性的强抑制子。
Nature. 1967 Apr 29;214(5087):452-3. doi: 10.1038/214452a0.
10
Nonsense codons and polarity in the tryptophan operon.色氨酸操纵子中的无义密码子与极性
J Mol Biol. 1966 Nov 14;21(2):313-34. doi: 10.1016/0022-2836(66)90102-1.
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