纳豆（发酵大豆）生产用枯草芽孢杆菌菌株的系统发育分析。
Phylogenetic analysis of Bacillus subtilis strains applicable to natto (fermented soybean) production.
机构信息
Industrial Technology Institute of Ibaraki Prefecture, Nagaoka, Ibarakimachi, Ibaraki, Japan.
出版信息
Appl Environ Microbiol. 2011 Sep;77(18):6463-9. doi: 10.1128/AEM.00448-11. Epub 2011 Jul 15.
Abstract
Spore-forming Bacillus strains that produce extracellular poly-γ-glutamic acid were screened for their application to natto (fermented soybean food) fermentation. Among the 424 strains, including Bacillus subtilis and B. amyloliquefaciens, which we isolated from rice straw, 59 were capable of fermenting natto. Biotin auxotrophism was tightly linked to natto fermentation. A multilocus nucleotide sequence of six genes (rpoB, purH, gyrA, groEL, polC, and 16S rRNA) was used for phylogenetic analysis, and amplified fragment length polymorphism (AFLP) analysis was also conducted on the natto-fermenting strains. The ability to ferment natto was inferred from the two principal components of the AFLP banding pattern, and natto-fermenting strains formed a tight cluster within the B. subtilis subsp. subtilis group.
摘要
我们从稻草中分离到的 424 株包括枯草芽孢杆菌和解淀粉芽孢杆菌在内的芽孢杆菌菌株中，筛选出能够发酵纳豆的菌株，其中 59 株能够发酵纳豆。生物素营养缺陷型与纳豆发酵紧密相关。利用 6 个基因（rpoB、purH、gyrA、groEL、polC 和 16S rRNA）的多位点核苷酸序列进行系统发育分析，并对纳豆发酵菌株进行扩增片段长度多态性（AFLP）分析。从 AFLP 带型的两个主成分推断出发酵纳豆的能力，纳豆发酵菌株在枯草芽孢杆菌亚种枯草芽孢杆菌群内形成一个紧密的聚类。
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