Matsui Toru, Maruhashi Kenji
Bio-Refining Process Laboratory, Japan Cooperation Center, Petroleum, 1900 Sodeshi-cho Shimizu-shi, Shizuoka 424-0037, Japan.
Curr Microbiol. 2004 Feb;48(2):130-4. doi: 10.1007/s00284-003-4141-2.
The dibenzothiophene-desulfurizing nocardioform bacteria, Gordonia sp. TM414, was isolated from oil-contaminated soil. To avoid coloration of the oil layer after the desulfurization reaction, which could decrease the quality of the oil, two colorless knock-out mutants, TPc and TPd, were isolated by using a broad-host-range transposon complex. Genomic sequence analysis revealed that the same gene was disrupted in these mutants and that the transposon-inserted gene was assigned as the gene for phytoene desaturase, crt I. The crt I mutants also showed desulfurization activity comparable to that of the parent strain in a model-oil/aqueous bi-phasic reaction, suggesting that the carotenoid production is not responsible for the bi-phasic desulfurization reaction that requires hydrophobic substrate incorporation from the organic phase.
从受油污染的土壤中分离出了具有二苯并噻吩脱硫能力的诺卡氏菌属细菌戈登氏菌TM414。为避免脱硫反应后油层变色从而降低油的质量，利用广宿主范围转座子复合体分离出了两个无色敲除突变体TPc和TPd。基因组序列分析表明，这些突变体中相同的基因被破坏，并且转座子插入的基因被确定为八氢番茄红素去饱和酶基因crt I。在模型油/水双相反应中，crt I突变体的脱硫活性也与亲本菌株相当，这表明类胡萝卜素的产生与需要从有机相中掺入疏水底物的双相脱硫反应无关。
