Drzyzga Oliver, Navarro Llorens J María, Fernández de Las Heras Laura, García Fernández Esther, Perera Julián
Departamento de Bioquímica y Biología Molecular I, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Int J Syst Evol Microbiol. 2009 May;59(Pt 5):1011-5. doi: 10.1099/ijs.0.005777-0.
The taxonomic position of the cholesterol-degrading strain Chol-3(T), isolated from a sewage sludge sample, was clarified using a polyphasic taxonomic approach. Phylogenetic analysis of its 16S rRNA gene sequence, whole-cell fatty acid profile and mycolic acid composition revealed that this isolate is a member of the genus Gordonia with the species Gordonia sihwensis, G. hydrophobica and G. shandongensis being the nearest phylogenetic neighbours. The results of DNA-DNA hybridization against its phylogenetically closest neighbours as well as the results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Chol-3(T) from the other Gordonia species with validly published names. Strain Chol-3(T) therefore merits recognition as a member of a novel species within the genus Gordonia, for which the name Gordonia cholesterolivorans sp. nov. is proposed. The type strain is Chol-3(T) (=CECT 7408(T) =DSM 45229(T)).
采用多相分类学方法阐明了从污水污泥样品中分离出的胆固醇降解菌株Chol-3(T)的分类地位。对其16S rRNA基因序列、全细胞脂肪酸谱和分枝菌酸组成进行系统发育分析，结果表明该分离株属于戈登氏菌属，与嗜水戈登氏菌、疏水戈登氏菌和山东戈登氏菌是最近的系统发育邻种。对其系统发育关系最密切的近缘种进行DNA-DNA杂交的结果以及生理生化测试的结果，使得Chol-3(T)菌株能够在基因型和表型上与其他已有效发表名称的戈登氏菌物种区分开来。因此，Chol-3(T)菌株应被认定为戈登氏菌属内一个新物种的成员，为此提出新名称戈登氏胆固醇降解菌。模式菌株为Chol-3(T)(=CECT 7408(T)=DSM 45229(T))。
