采用微透析-高效液相色谱法测定银杏叶提取物中银杏内酯A、B及白果内酯的含量。
Determination of ginkgolide A, B, and bilobalide in biloba L. extracts by microdialysis-HPLC.
作者信息
Chiu Houn-Lin, Lin Hui-Ya, Yang Thomas Ching-Cherng
机构信息
Department of Chemistry, National Kaohsiung Normal University, 116 Ho-Ping 1st Rd., Kaohsiung, Taiwan.
出版信息
Anal Bioanal Chem. 2004 Jun;379(3):445-8. doi: 10.1007/s00216-004-2606-1. Epub 2004 Apr 1.
A method has been developed for measuring ginkgolide A, B, and bilobalide in ginkgo biloba L. extracts by using a self-assembled microdialysis device coupled on-line to high-performance liquid chromatography. The optimal conditions for dialysis efficiency and chromatographic analysis were investigated. The results show that the dialysis efficiencies for ginkgolide A, B, and bilobalide are 97.8-100.4%. For the spike concentration of 45 mg L(-1), the recoveries of ginkgolide A, B, and bilobalide in ginkgo biloba L. extracts were at 97-100.7% with 3.1-4.7% RSD. The proposed method has therefore been shown to be applicable to the analysis of ginkgolide A, B, and bilobalide in ginkgo biloba L. extracts.
已开发出一种方法，通过使用与高效液相色谱在线联用的自组装微透析装置来测定银杏叶提取物中的银杏内酯A、B和白果内酯。研究了透析效率和色谱分析的最佳条件。结果表明，银杏内酯A、B和白果内酯的透析效率为97.8 - 100.4%。对于45 mg L(-1)的加标浓度，银杏叶提取物中银杏内酯A、B和白果内酯的回收率为97 - 100.7%，相对标准偏差为3.1 - 4.7%。因此，所提出的方法已被证明适用于银杏叶提取物中银杏内酯A、B和白果内酯的分析。
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