Jaracz Stanislav, Malik Shahid, Nakanishi Koji
Department of Chemistry, Columbia University, 3000 Broadway, NY 10027, USA.
Phytochemistry. 2004 Nov;65(21):2897-902. doi: 10.1016/j.phytochem.2004.08.026.
Ginkgolides A, B, C and J, together with bilobalide, are unique terpenoid components of the Ginkgo biloba tree. Due to similar chemical properties, their separation is quite tedious. We have developed an efficient and rapid protocol for separation of individual ginkgolides and bilobalide from G. biloba extracts. The procedure takes advantage of enhanced susceptibility of ginkgolides B and C to benzylation and the ease of separation of these products from ginkgolides A and J which do not react. The protocol is applicable to the previously reported enriched extracts prepared from G. biloba leaves. A single chromatographic step prior to benzylation provides bilobalide and mixture of ginkgolides A, B, C, and J. After benzylation, the individual ginkgolides are separated by chromatography.
银杏内酯A、B、C和J以及白果内酯是银杏树特有的萜类成分。由于化学性质相似，它们的分离相当繁琐。我们开发了一种高效快速的方法，用于从银杏提取物中分离出单一的银杏内酯和白果内酯。该方法利用了银杏内酯B和C对苄基化增强的敏感性，以及这些产物与不发生反应的银杏内酯A和J易于分离的特点。该方法适用于先前报道的从银杏叶制备的富集提取物。在苄基化之前进行一步色谱分离可得到白果内酯以及银杏内酯A、B、C和J的混合物。苄基化之后，通过色谱法分离出单一的银杏内酯。
