具有独特基因组特征的结核分枝杆菌分离株过表达一种类似tap的外排泵。
Mycobacterium tuberculosis isolate with a distinct genomic identity overexpresses a tap-like efflux pump.
作者信息
Siddiqi N, Das R, Pathak N, Banerjee S, Ahmed N, Katoch V M, Hasnain S E
机构信息
Centre for DNA Fingerprinting and Diagnostics, Nacharam, Hyderabad, India.
出版信息
Infection. 2004 Apr;32(2):109-11. doi: 10.1007/s15010-004-3097-x.
BACKGROUND
One mechanism proposed for drug resistance in Mycobacterium tuberculosis (MTB) is by efflux of the drugs by membrane located pumps. We report a novel and definite association between drug resistance and transcription levels of a tap-like pump (Rv1258c) in a multi-drug resistant MTB patient isolate (ICC154) which possesses a unique genotypic signature.
MATERIALS AND METHODS
The isolate ICC154 was tested for drug sensitivity. Over-expression of Rv1258c as a function of drug pressure was analyzed by RT-PCR and the strain was typed using fluorescent amplified fragment length polymorhism (FAFLP).
RESULT
In the presence of rifampicin and ofloxacin, this isolate shows increased transcription of the gene Rv1258c. Genotypic fingerprinting revealed the presence of unique FAFLP markers.
CONCLUSION
A clear association between drug resistance and overexpression of an efflux protein is evident from our studies. The presence of specific markers has implications in rapid identification of MDR clinical isolates and consequent disease management.
背景
结核分枝杆菌（MTB）耐药的一种机制是通过位于细胞膜上的泵将药物外排。我们报告了在一株具有独特基因型特征的耐多药MTB患者分离株（ICC154）中，耐药性与一种类tap泵（Rv1258c）转录水平之间存在一种新的明确关联。
材料与方法
对分离株ICC154进行药敏试验。通过逆转录聚合酶链反应（RT-PCR）分析Rv1258c作为药物压力函数的过表达情况，并使用荧光扩增片段长度多态性（FAFLP）对该菌株进行分型。
结果
在利福平和氧氟沙星存在的情况下，该分离株显示出Rv1258c基因转录增加。基因型指纹图谱显示存在独特的FAFLP标记。
结论
我们的研究表明耐药性与外排蛋白过表达之间存在明显关联。特定标记的存在对快速鉴定耐多药临床分离株及后续疾病管理具有重要意义。
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