Lindeman J H, Houdkamp E, Lentjes E G, Poorthuis B J, Berger H M
Department of Pediatrics, University Hospital, Leiden, The Netherlands.
Free Radic Res Commun. 1992;16(5):285-94. doi: 10.3109/10715769209049181.
The ability of plasma from newborn babies (cord blood) and adults to inhibit iron-induced lipid peroxidation was compared. The caeruloplasmin and transferrin concentrations, and latent iron-binding capacity were lower in the babies (p less than 0.001). The plasma of many of the babies had no latent iron-binding capacity and contained non-protein-bound iron (measured by the bleomycin assay). The in vitro ability of plasma to inhibit iron-induced liposome peroxidation by either ferroxidase antioxidant activity (caeruloplasmin) or iron-binding antioxidant activity (transferrin) was measured. The antioxidant activity in both assays was decreased in the babies (p less than 0.001). The percentage inhibition of peroxidation in the iron-binding antioxidant assay correlated positively with the latent iron-binding capacity (p less than 0.001) and negatively with the presence of bleomycin-detectable iron (p less than 0.02) in the babies. This assay produced stimulation of peroxidation in 42% of the babies but none of the adults. The diminished capacity of cord blood plasma to prevent iron-induced lipid peroxidation may predispose the newborn baby to the toxic effects of oxygen.
比较了新生儿（脐带血）和成年人血浆抑制铁诱导的脂质过氧化的能力。婴儿血浆中的铜蓝蛋白和转铁蛋白浓度以及潜在铁结合能力较低（p<0.001）。许多婴儿的血浆没有潜在铁结合能力，且含有非蛋白结合铁（通过博来霉素检测法测量）。测定了血浆通过铁氧化酶抗氧化活性（铜蓝蛋白）或铁结合抗氧化活性（转铁蛋白）在体外抑制铁诱导脂质体过氧化的能力。两种检测中的抗氧化活性在婴儿中均降低（p<0.001）。在婴儿中，铁结合抗氧化检测中过氧化的抑制百分比与潜在铁结合能力呈正相关（p<0.001），与博来霉素可检测到的铁的存在呈负相关（p<0.02）。该检测在42%的婴儿中产生了过氧化刺激，但在成年人中未出现。脐带血血浆预防铁诱导脂质过氧化的能力减弱可能使新生儿易受氧的毒性作用影响。
