Jansen Koen, Meek Marcel F, van der Werff John F A, van Wachem Pauline B, van Luyn Marja J A
Department of Plastic Surgery, University Hospital Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
J Biomed Mater Res A. 2004 May 1;69(2):334-41. doi: 10.1002/jbm.a.30004.
Long-term studies on nerve-guide regeneration are scarce. Therefore, in rats, long-term (16 months) sciatic nerve regeneration through poly(DL-lactide-epsilon-caprolactone) [poly(DLLA-epsilon-CL)] nerve guides was studied and compared with the nonoperated control side. Poly(DLLA-epsilon-CL) degradation and possible long-term foreign body reaction against poly(DLLA-epsilon-CL) nerve guides, as well as the distribution of both collagen type III and IV were studied. In vivo poly(DLLA-epsilon-CL) studies have been performed before but not for such long time points; also, a detailed analysis of collagen III/IV has not been presented before. The results demonstrate that biodegradable poly(DLLA-epsilon-CL) nerve guides yield good nerve regeneration and collagen III/IV deposition relative to the anatomy of the control side. Regenerated nerve showed almost similar collagen type III/IV distribution patterns as compared with the nonoperated control side, although the delineation of matrix was clearer in the control side. The relative amount of collagen III and IV immunostaining in nerve cross-sections did not, however, differ between the control nerve tissue and the operated side after 16 months. After 16 months of implantation, however, some very small fragments of biomaterial could still be found on the edge of the epineurium of the regenerated nerve, indicating remnants of a secondary foreign body reaction. The biomaterial fragments and foreign body reaction did not influence the nerve regeneration process after 16 months. Biodegradable poly(DLLA-epsilon-CL) nerve guides are useful for long-term bridging of short peripheral nerve gaps.
关于神经导管再生的长期研究很少。因此，在大鼠中，研究了聚（DL-丙交酯-ε-己内酯）[聚（DLLA-ε-CL）]神经导管介导的坐骨神经长期（16个月）再生，并与未手术的对照侧进行比较。研究了聚（DLLA-ε-CL）的降解情况、对聚（DLLA-ε-CL）神经导管可能的长期异物反应，以及III型和IV型胶原蛋白的分布。之前已经进行过聚（DLLA-ε-CL）的体内研究，但时间点没有这么长；此外，之前也没有对胶原蛋白III/IV进行详细分析。结果表明，相对于对照侧的解剖结构，可生物降解的聚（DLLA-ε-CL）神经导管能实现良好的神经再生和胶原蛋白III/IV沉积。与未手术的对照侧相比，再生神经的III型/IV型胶原蛋白分布模式几乎相似，尽管对照侧的基质轮廓更清晰。然而，16个月后，对照神经组织和手术侧神经横断面中胶原蛋白III和IV免疫染色的相对量没有差异。然而，植入16个月后，在再生神经的神经外膜边缘仍可发现一些非常小的生物材料碎片，表明存在继发性异物反应的残余物。16个月后，生物材料碎片和异物反应并未影响神经再生过程。可生物降解的聚（DLLA-ε-CL）神经导管可用于短期周围神经缺损的长期桥接。
