Campanero Miguel Angel, Cardenas Ernesto, Sádaba Belén, García-Quetglas Emilio, Muñoz-Juarez Maria Jose, Gil-Aldea Isabel, Pazo David, Azanza Jose Ramón, Honorato Jesús
Servicio de Farmacología Clínica, Clínica Universitaria de Navarra, 31080, C/Pio XII s/n. 31008 Pamplona (Navarra), Spain.
J Chromatogr A. 2004 Mar 26;1031(1-2):265-73. doi: 10.1016/j.chroma.2003.10.121.
We developed and validated an accurate, sensitive, precise and rapid HPLC method with UV detection for the determination of sirolimus in blood samples from renal, cardiac and hepatic transplants. This method overcomes most of the problems related to previously published assays using a narrow-bore column with base deactivated C18 reversed phase. Whole blood samples were purified by a combination of a precipitating blood matrix with zinc sulphate and a single step liquid-liquid extraction with acetone and 1-chlorobutane. Calibration curves (range 2.5-150 ng/ml), were linear with coefficients of correlation better than 0.996. The relative standard deviation was determined to be less than 8%. The present method has also been validated by a reference laboratory (St. George's Hospital Medical School, London, UK). More of 300 clinical samples have been analysed with this method.
