采用紫外检测的高效液相色谱法用于依维莫司的治疗药物监测。
High-performance liquid chromatography with ultraviolet detection for therapeutic drug monitoring of everolimus.
作者信息
Baldelli Sara, Murgia Stefano, Merlini Simona, Zenoni Stefania, Perico Norberto, Remuzzi Giuseppe, Cattaneo Dario
机构信息
Department of Medicine and Transplantation, Ospedali Riuniti di Bergamo-Mario Negri Institute for Pharmacological Research, Via Gavazzeni 11, 24125 Bergamo, Italy.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Feb 25;816(1-2):99-105. doi: 10.1016/j.jchromb.2004.11.016.
We developed and validated a high-performance liquid chromatography-ultraviolet (HPLC-UV) method for determining everolimus concentrations in human whole blood. Sample preparation involved a solid-phase extraction after protein precipitation. The separation of everolimus from internal standard (IS) and endogenous components was achieved using an isocratic elution on an octyl column. The method showed a linear relationship between peak height ratios and blood concentrations in the range of 1-200 ng/mL (r(2)=0.9997). The observed intra- and inter-day assay imprecision had a coefficient of variation (CV)=12.8%, and inaccuracy was 11.4%. The method was found to be precise, accurate, and sensible making it useful for routine therapeutic monitoring of everolimus.
我们开发并验证了一种用于测定人全血中依维莫司浓度的高效液相色谱 - 紫外（HPLC - UV）方法。样品制备包括蛋白质沉淀后的固相萃取。使用辛基柱等度洗脱实现了依维莫司与内标（IS）及内源性成分的分离。该方法在1 - 200 ng/mL范围内峰高比与血药浓度呈线性关系（r(2)=0.9997）。观察到的日内和日间测定不精密度的变异系数（CV）=12.8%，不准确率为11.4%该方法被发现具有精密度、准确性和灵敏度，使其可用于依维莫司的常规治疗监测。
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