[RhoA在人胃癌细胞系中的表达及可能的功能]
[The expression and possible function of RhoA in human gastric cancer cell lines].
作者信息
Liu Na, Bi Feng, Pan Yang-lin, Xue Yan, Zhang Xing, Shi Yong-quan, Zhang Yu-mei, Du Jing-ping, Fan Dai-ming
机构信息
Institute of Digestive Diseases, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, China.
出版信息
Zhonghua Zhong Liu Za Zhi. 2004 Jan;26(1):26-9.
OBJECTIVE
To study the expression and possible function of RhoA in human gastric cancer cell lines.
METHODS
The expression of RhoA in human gastrointestinal cancer cell lines was detected by Western blot. Antisense plasmid of RhoA was constructed by pGEFL and transferred into gastric cancer cell line AGS by lipofectamine. Cell survival was examined by MTT assays, and cell cycle was detected by flow cytometry.
RESULTS
The expression of RhoA protein in 10 different kinds of human cancer cell lines was much higher than that in immortalized human intestinal epithelial cell line. After being transfected with antisense RhoA, with the decrease in RhoA protein expression, the growth rate of AGS was inhibited, and the number of cells in S phase was increased by 14%.
CONCLUSION
RhoA is overexpressed in many human cancer cell lines. Some of the malignant characteristics of a gastric cancer cell line can be partially reversed by inhibiting RhoA expression.
目的
研究RhoA在人胃癌细胞系中的表达及可能的功能。
方法
采用蛋白质免疫印迹法检测RhoA在人胃肠道癌细胞系中的表达。利用pGEFL构建RhoA反义质粒，并通过脂质体转染法将其转入胃癌细胞系AGS。采用MTT法检测细胞存活率，通过流式细胞术检测细胞周期。
结果
10种不同的人癌细胞系中RhoA蛋白的表达明显高于永生化人肠上皮细胞系。转染反义RhoA后，随着RhoA蛋白表达的降低，AGS细胞的生长速率受到抑制，S期细胞数量增加了14%。
结论
RhoA在多种人癌细胞系中过度表达。抑制RhoA的表达可部分逆转胃癌细胞系的某些恶性特征。
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