黄杆菌耐药性的分离分析及其β-内酰胺酶的产生
[Isolation and analysis of the drug resistance of the flavobacterium and its production of beta-lactamases].
作者信息
Luo Yu, Xia Pei-yuan, Wang Xian-yuan, Yin Chun-hong
机构信息
National Base for Drug Clinical Trial, Southwest Hospital, The Third Military Medical University, Chongqing, 400038. P.R. China.
出版信息
Zhonghua Shao Shang Za Zhi. 2004 Feb;20(1):14-6.
OBJECTIVE
To investigate the drug resistance of flavobacterium and its ability to produce BLA (beta-lactamases) and ESBLs (Extended-spectrum beta-lactamases).
METHODS
The production of BLA and ESBLs from 6 clinical isolated flavobacterium strains was determined by nitrocefin disc test and double-disc synergy method, respectively. The antibiotic susceptibilities of the strains were determined by Kirby-Bauer disc diffusion test and the agar dilution method and the MIC was assessed.
RESULTS
All the six flavobacteria were BLA-producing strains and more than 80% of them were ESBLs-producing, and they were highly resistant to beta-lactamase antibiotics (MIC 32 - 256 mg/L), but susceptible to fluoroquinolones and cephalosporin with beta-lactamase inhibitors (MIC 0.125 - 8 mg/L).
CONCLUSION
Most of the flavobacteria in nosocomial infections were beta-lactamase-producing and were highly resistant to beta-lactamase antibiotics. Fluoroquinolones and beta-lactamase antibiotics with lactamase inhibitors should be the first choice for the management of infection caused by flavobacterium.
目的
研究黄杆菌的耐药性及其产生β-内酰胺酶（BLA）和超广谱β-内酰胺酶（ESBLs）的能力。
方法
分别采用头孢硝噻吩纸片法和双纸片协同法检测6株临床分离黄杆菌菌株BLA和ESBLs的产生情况。采用 Kirby-Bauer 纸片扩散法和琼脂稀释法测定菌株的抗生素敏感性并评估最低抑菌浓度（MIC）。
结果
所有6株黄杆菌均为产BLA菌株，其中80%以上为产ESBLs菌株，它们对β-内酰胺酶抗生素高度耐药（MIC为32 - 256 mg/L），但对氟喹诺酮类药物和含β-内酰胺酶抑制剂的头孢菌素敏感（MIC为0.125 - 8 mg/L）。
结论
医院感染中的大多数黄杆菌产β-内酰胺酶，对β-内酰胺酶抗生素高度耐药。氟喹诺酮类药物和含β-内酰胺酶抑制剂的β-内酰胺类抗生素应作为治疗黄杆菌所致感染的首选药物。
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