夜猴CSF1R和IL5基因的染色体定位

Chromosome mapping of the owl monkey CSF1R and IL5 genes.

作者信息

Ma N S, Lin K C

机构信息

Department of Pathology, New England Regional Primate Research Center, Southborough, Massachusetts 01772-9102.

出版信息

Genomics. 1992 Aug;13(4):1174-7. doi: 10.1016/0888-7543(92)90034-p.

DOI:10.1016/0888-7543(92)90034-p

PMID:1505950

Abstract

We mapped the owl monkey colony-stimulating factor 1 receptor (CSF1R) locus to the proximal region of chromosome 3q of karyotype VI(K-VI) and karyotype V(K-V) and the interleukin 5 (IL5) locus to the mid-region of chromosome 3q(K-VI) and 19q(K-IV) using a combination of Southern hybridization of somatic cells and in situ chromosomal hybridization methodologies. The findings support the proposed evolution of owl monkey chromosome 3(K-VI) from a fusion of two smaller structures, the homologs of chromosomes 6 and 19 (K-IV). The data also indicate genomic conservation of the HSA 5q23-q35 segment in the higher primates.

摘要

我们采用体细胞Southern杂交和原位染色体杂交方法相结合的方式，将夜猴集落刺激因子1受体（CSF1R）基因座定位到核型VI（K-VI）和核型V（K-V）的3号染色体3q近端区域，将白细胞介素5（IL5）基因座定位到核型VI（K-VI）的3号染色体3q中间区域以及核型IV（K-IV）的19号染色体19q中间区域。这些发现支持了夜猴3号染色体（K-VI）由两个较小结构（6号和19号染色体（K-IV）的同源染色体）融合而来的进化假说。数据还表明了人5号染色体5q23-q35区段在高等灵长类动物中的基因组保守性。