Park Eung Jo, Bae Young Chan
Division of Chemical Engineering, Molecular Thermodynamics Laboratory, Hanyang University, Sungdonggu, Seoul 133-791, South Korea.
Biophys Chem. 2004 Apr 1;109(1):169-88. doi: 10.1016/j.bpc.2003.11.001.
Liquid-liquid phase-separation data are obtained for aqueous solutions of lysozyme. Thermooptical analysis (TOA) technique overcomes many defects of the light scattering method, which is most commonly used for this purpose, and provides a simple, rapid and reliable experimental method to determine cloud-point temperatures (CPTs) of aqueous protein solution systems. The TOA apparatus described here needs very small amount of samples (0.02 ml), and CPT can easily be determined in a very short time. The CPTs are measured as a function of salt type and concentration at pH 4.0 and 7.0. Salts used include those from mono and divalent cations and anions, and the modified Perturbed-Hard-Sphere-Chain (PHSC) model that takes into account the shape of protein is used to interpret the effect of salts.
获得了溶菌酶水溶液的液-液相分离数据。热光分析（TOA）技术克服了为此目的最常用的光散射方法的许多缺陷，并提供了一种简单、快速且可靠的实验方法来测定蛋白质水溶液系统的浊点温度（CPT）。这里描述的TOA仪器需要非常少量的样品（0.02毫升），并且可以在很短的时间内轻松测定CPT。在pH值为4.0和7.0的条件下，测量CPT随盐类型和浓度的变化。所使用的盐包括单价和二价阳离子及阴离子的盐，并且使用考虑了蛋白质形状的修正扰动硬球链（PHSC）模型来解释盐的作用。
