Department of Chemistry and CSGI, University of Florence, 50019 Sesto Fiorentino, Florence, Italy.
J Am Chem Soc. 2010 May 12;132(18):6571-7. doi: 10.1021/ja101603n.
An aqueous dispersion of lysozyme and sodium dodecyl sulfate phase separates below the cloud point. Two liquid phases are formed. At pH < pI electrolytes change the cloud point temperatures (T(c)). These follow an inverse Hofmeister series. Anions partition asymmetrically between the two phases. At fixed cation, the partitioning depends on the specific anion. In a system of finite volume, a concentrated dispersion of a protein is able to act as a chemical "sponge". No active pump is necessary to maintain concentration differences between concentrated and dilute dispersions.
溶菌酶和十二烷基硫酸钠的水溶液在浊点以下相分离。形成两相。在 pH < pI 时，电解质改变浊点温度 (T(c))。这些遵循反霍夫迈斯特序列。阴离子在两相之间不对称分配。在固定阳离子的情况下，分配取决于特定的阴离子。在有限体积的系统中，浓缩的蛋白质分散体能够充当化学“海绵”。不需要主动泵来维持浓缩和稀释分散体之间的浓度差异。
Zotero 插件