利用高效液相色谱法鉴定和纯化人类X染色体Xq24-qter区域的重叠黏粒克隆。

Identification and purification of overlapping cosmid clones of the region Xq24-qter of human X chromosome using HPLC.

作者信息

Ida B, Pierluigi M, Lucia S, Piergiorgio P

机构信息

Istituto Tecnologie Biomediche Avanzate, Milan, Italy.

出版信息

Genomics. 1992 Aug;13(4):1353-5. doi: 10.1016/0888-7543(92)90068-4.

DOI:10.1016/0888-7543(92)90068-4

PMID:1505974

Abstract

The assembly of a large physical map of genomes requires simultaneous analysis of many cosmid clones for overlapping regions. The search for overlapping regions may be achieved by various means. High-performance liquid chromatography (HPLC) provides an alternative to gel electrophoresis since microgram amounts of each DNA fragment may be collected into individual test tubes for further analysis. HPLC has been used to identify overlapping cosmid clones from a pool of cosmid DNA containing the terminal portion of the long arm of the human X chromosome (Xq24-qter). Among 400 cosmids analyzed, 3 were shown to overlap.

摘要

构建基因组的大型物理图谱需要同时分析许多黏粒克隆的重叠区域。寻找重叠区域可以通过多种方式实现。高效液相色谱法（HPLC）为凝胶电泳提供了一种替代方法，因为可以将微克量的每个DNA片段收集到单独的试管中进行进一步分析。HPLC已被用于从包含人类X染色体长臂末端部分（Xq24-qter）的黏粒DNA池中鉴定重叠的黏粒克隆。在分析的400个黏粒中，有3个显示出重叠。