随机挑选的黏粒克隆覆盖了大肠杆菌染色体物理图谱中的pyrB和oriC缺口。
Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E. coli chromosome.
作者信息
Knott V, Rees D J, Cheng Z, Brownlee G G
机构信息
Sir William Dunn School of Pathology, University of Oxford, UK.
出版信息
Nucleic Acids Res. 1988 Mar 25;16(6):2601-12. doi: 10.1093/nar/16.6.2601.
Abstract
Sets of overlapping cosmid clones generated by random sampling and fingerprinting methods complement data at pyrB (96.5') and oriC (84') in the published physical map of E. coli. A new cloning strategy using sheared DNA, and a low copy, inducible cosmid vector were used in order to reduce bias in libraries, in conjunction with micro-methods for preparing cosmid DNA from a large number of clones. Our results are relevant to the design of the best approach to the physical mapping of large genomes.
摘要
通过随机抽样和指纹图谱法产生的重叠黏粒克隆集补充了已发表的大肠杆菌物理图谱中pyrB（96.5'）和oriC（84'）处的数据。为了减少文库中的偏差，采用了一种使用剪切DNA的新克隆策略以及一种低拷贝、可诱导的黏粒载体，并结合从大量克隆中制备黏粒DNA的微量方法。我们的结果与大基因组物理图谱绘制的最佳方法设计相关。
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本文引用的文献
1
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Proc Natl Acad Sci U S A. 1986 Oct;83(20):7821-5. doi: 10.1073/pnas.83.20.7821.
2
A general method for preparing intact nuclear DNA.一种制备完整核DNA的通用方法。
EMBO J. 1984 Aug;3(8):1837-42. doi: 10.1002/j.1460-2075.1984.tb02056.x.
3
DNA sequence around the Escherichia coli unc operon. Completion of the sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and phoS.大肠杆菌unc操纵子周围的DNA序列。包含天冬酰胺合成酶A（asnA）、复制起点（oriC）、unc、葡糖胺-6-磷酸合成酶（glmS）和磷酸酶S（phoS）的17千碱基片段序列的完成。
Biochem J. 1984 Dec 15;224(3):799-815. doi: 10.1042/bj2240799.
4
Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.通过脉冲场梯度凝胶电泳分离酵母染色体大小的DNA。
Cell. 1984 May;37(1):67-75. doi: 10.1016/0092-8674(84)90301-5.
5
Low-copy-number plasmid-cloning vectors amplifiable by derepression of an inserted foreign promoter.可通过插入的外源启动子去阻遏作用进行扩增的低拷贝数质粒克隆载体。
Gene. 1984 Apr;28(1):45-54. doi: 10.1016/0378-1119(84)90086-6.
6
The gene structure of human anti-haemophilic factor IX.人抗血友病因子IX的基因结构
EMBO J. 1984 May;3(5):1053-60. doi: 10.1002/j.1460-2075.1984.tb01926.x.
7
The construction of cosmid libraries which can be used to transform eukaryotic cells.可用于转化真核细胞的黏粒文库的构建。
Nucleic Acids Res. 1982 Nov 11;10(21):6715-32. doi: 10.1093/nar/10.21.6715.
8
Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.大肠杆菌产生的DNA的宿主特异性：影响DNA限制与修饰的细菌突变
J Mol Biol. 1966 Mar;16(1):118-33. doi: 10.1016/s0022-2836(66)80267-x.
9
Random-clone strategy for genomic restriction mapping in yeast.酵母基因组限制性图谱绘制的随机克隆策略。
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7826-30. doi: 10.1073/pnas.83.20.7826.
10
A physical map of the Escherichia coli K12 genome.大肠杆菌K12基因组的物理图谱。
Science. 1987 Jun 12;236(4807):1448-53. doi: 10.1126/science.3296194.
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