人类染色体端粒和脆性位点区域内C2-H2锌指基序序列的聚类。

Clustering of C2-H2 zinc finger motif sequences within telomeric and fragile site regions of human chromosomes.

作者信息

Lichter P, Bray P, Ried T, Dawid I B, Ward D C

机构信息

Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510.

出版信息

Genomics. 1992 Aug;13(4):999-1007. doi: 10.1016/0888-7543(92)90013-i.

DOI:10.1016/0888-7543(92)90013-i

PMID:1505991

Abstract

Ninety-three phage clones identified by hybridization with a C2-H2 zinc finger sequence probe have been grouped into 23 genetic loci. Partial sequencing verified that each locus belonged to the zinc finger family. Oligonucleotide primer pairs were developed from these sequences to serve as STS markers for these loci. One or more clones from each locus was mapped onto human metaphase chromosomes by fluorescence in situ hybridization. Several loci map to identical chromosomal regions, indicating the possible presence of multigene clusters. Zinc finger loci were found to reside predominantly either in telomeric regions or in chromosomal bands known to exhibit chromosome fragility. Chromosome 19 carries a disproportionate fraction (10 of 23) of the mapped zinc finger loci.

摘要

通过与C2-H2锌指序列探针杂交鉴定出的93个噬菌体克隆已被归为23个基因座。部分测序证实每个基因座都属于锌指家族。根据这些序列开发了寡核苷酸引物对，用作这些基因座的STS标记。通过荧光原位杂交将每个基因座的一个或多个克隆定位到人类中期染色体上。几个基因座定位于相同的染色体区域，表明可能存在多基因簇。发现锌指基因座主要位于端粒区域或已知表现出染色体脆性的染色体带中。19号染色体携带了不成比例的已定位锌指基因座（23个中的10个）。