Cfa1的特性，一种参与植物毒素冠菌素生物合成的单功能酰基载体蛋白。
Characterization of Cfa1, a monofunctional acyl carrier protein involved in the biosynthesis of the phytotoxin coronatine.
作者信息
Seidle Heather, Rangaswamy Vidhya, Couch Robin, Bender Carol L, Parry Ronald J
机构信息
Department of Chemistry, Rice University, Houston, Texas 77005, USA.
出版信息
J Bacteriol. 2004 Apr;186(8):2499-503. doi: 10.1128/JB.186.8.2499-2503.2004.
Abstract
Cfa1 was overproduced in Escherichia coli and Pseudomonas syringae, and the degree of 4'-phosphopantetheinylation was determined. The malonyl-coenzyme A:acyl carrier protein transacylase (FabD) of P. syringae was overproduced and shown to catalyze malonylation of Cfa1, suggesting that FabD plays a role in coronatine biosynthesis. Highly purified Cfa1 did not exhibit self-malonylation activity.
摘要
Cfa1在大肠杆菌和丁香假单胞菌中过量表达，并测定了4'-磷酸泛酰巯基乙胺化程度。丁香假单胞菌的丙二酰辅酶A：酰基载体蛋白转酰基酶（FabD）过量表达，并显示可催化Cfa1的丙二酰化，这表明FabD在冠菌素生物合成中发挥作用。高度纯化的Cfa1未表现出自我丙二酰化活性。
相似文献
1
Characterization of Cfa1, a monofunctional acyl carrier protein involved in the biosynthesis of the phytotoxin coronatine.Cfa1的特性，一种参与植物毒素冠菌素生物合成的单功能酰基载体蛋白。
J Bacteriol. 2004 Apr;186(8):2499-503. doi: 10.1128/JB.186.8.2499-2503.2004.
2
Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-coenzyme A:ACP transacylase (FabD).铜绿假单胞菌脂肪酸生物合成基因簇的表征：酰基载体蛋白（ACP）和丙二酰辅酶A：ACP转酰基酶（FabD）的纯化
J Bacteriol. 1999 Sep;181(17):5498-504. doi: 10.1128/JB.181.17.5498-5504.1999.
3
Cloning, nucleotide sequence, and expression of the Escherichia coli fabD gene, encoding malonyl coenzyme A-acyl carrier protein transacylase.编码丙二酸单酰辅酶A-酰基载体蛋白转酰基酶的大肠杆菌fabD基因的克隆、核苷酸序列及表达
J Bacteriol. 1992 May;174(9):2851-7. doi: 10.1128/jb.174.9.2851-2857.1992.
4
Cloning and nucleotide sequence of the fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli.大肠杆菌丙二酰辅酶A-酰基载体蛋白转酰基酶编码基因fabD的克隆与核苷酸序列分析
FEBS Lett. 1992 Mar 16;299(3):262-6. doi: 10.1016/0014-5793(92)80128-4.
5
Malonyl-coenzyme A:acyl carrier protein acyltransferase of Streptomyces glaucescens: a possible link between fatty acid and polyketide biosynthesis.浅绿链霉菌丙二酸单酰辅酶A：酰基载体蛋白酰基转移酶：脂肪酸与聚酮化合物生物合成之间的可能联系。
Biochemistry. 1995 Jul 25;34(29):9389-402. doi: 10.1021/bi00029a015.
6
The R117A variant of the Escherichia coli transacylase FabD synthesizes novel acyl-(acyl carrier proteins).大肠杆菌转酰基酶 FabD 的 R117A 变体合成新型酰基-(酰基载体蛋白)。
Appl Microbiol Biotechnol. 2017 Dec;101(23-24):8431-8441. doi: 10.1007/s00253-017-8586-9. Epub 2017 Oct 26.
7
Characterisation of genes involved in biosynthesis of coronafacic acid, the polyketide component of the phytotoxin coronatine.冠毒素的聚酮成分冠菌酸生物合成相关基因的表征
Gene. 1996 Dec 12;183(1-2):167-73. doi: 10.1016/s0378-1119(96)00550-1.
8
Polyketide synthase acyl carrier protein (ACP) as a substrate and a catalyst for malonyl ACP biosynthesis.聚酮合酶酰基载体蛋白（ACP）作为丙二酸单酰ACP生物合成的底物和催化剂。
Chem Biol. 1999 Aug;6(8):577-84. doi: 10.1016/S1074-5521(99)80090-8.
9
A continuous coupled enzyme assay for bacterial malonyl-CoA:acyl carrier protein transacylase (FabD).一种用于细菌丙二酰辅酶A：酰基载体蛋白转酰基酶（FabD）的连续偶联酶测定法。
Anal Biochem. 2003 Aug 1;319(1):171-6. doi: 10.1016/s0003-2697(03)00327-0.
10
Cloning, expression, characterization, and interaction of two components of a human mitochondrial fatty acid synthase. Malonyltransferase and acyl carrier protein.人线粒体脂肪酸合酶两个组分的克隆、表达、特性鉴定及相互作用。丙二酰转移酶和酰基载体蛋白。
J Biol Chem. 2003 Oct 10;278(41):40067-74. doi: 10.1074/jbc.M306121200. Epub 2003 Jul 25.
引用本文的文献
1
Discovery of type II polyketide synthase-like enzymes for the biosynthesis of cispentacin.发现用于合成顺式戊烯二菌素的 II 型聚酮合酶样酶。
Nat Commun. 2023 Dec 6;14(1):8065. doi: 10.1038/s41467-023-43731-z.
2
Coronatine Gene Expression In Vitro and In Planta, and Protein Accumulation During Temperature Downshift in Pseudomonas syringae.拟南芥上的冠菌素基因表达体外和体内，以及在温度下降时在丁香假单胞菌中的蛋白积累。
Sensors (Basel). 2009;9(6):4272-85. doi: 10.3390/s90604272. Epub 2009 Jun 3.
本文引用的文献
1
Conservation of Plasmid DNA Sequences in Coronatine-Producing Pathovars of Pseudomonas syringae.冠菌素产生型丁香假单胞菌中质粒 DNA 序列的保守性。
Appl Environ Microbiol. 1991 Apr;57(4):993-9. doi: 10.1128/aem.57.4.993-999.1991.
2
Two simple media for the demonstration of pyocyanin and fluorescin.两种用于展示绿脓菌素和荧光素的简单培养基。
J Lab Clin Med. 1954 Aug;44(2):301-7.
3
Cloning and characterization of a polyketide synthase gene cluster involved in biosynthesis of a proposed angucycline-like polyketide auricin in Streptomyces aureofaciens CCM 3239.金色链霉菌CCM 3239中参与一种拟安古环类聚酮化合物奥瑞金生物合成的聚酮合酶基因簇的克隆与表征
Gene. 2002 Sep 4;297(1-2):197-208. doi: 10.1016/s0378-1119(02)00889-2.
4
Characterization of a new type of phosphopantetheinyl transferase for fatty acid and siderophore synthesis in Pseudomonas aeruginosa.铜绿假单胞菌中一种用于脂肪酸和铁载体合成的新型磷酸泛酰巯基乙胺转移酶的特性分析
J Biol Chem. 2002 Dec 27;277(52):50293-302. doi: 10.1074/jbc.M205042200. Epub 2002 Oct 14.
5
Enzymes involved in fatty acid and polyketide biosynthesis in Streptomyces glaucescens: role of FabH and FabD and their acyl carrier protein specificity.参与浅绿链霉菌脂肪酸和聚酮化合物生物合成的酶：FabH和FabD的作用及其酰基载体蛋白特异性
Biochemistry. 2002 Aug 20;41(33):10462-71. doi: 10.1021/bi0258804.
6
Construction of expression vectors to produce affinity-tagged proteins in Pseudomonas.构建用于在假单胞菌中产生亲和标签蛋白的表达载体。
Biotechniques. 2002 Jun;32(6):1230, 1232, 1234, 1236. doi: 10.2144/02326bm03.
7
Control of temperature-responsive synthesis of the phytotoxin coronatine in Pseudomonas syringae by the unconventional two-component system CorRPS.丁香假单胞菌中非常规双组分系统CorRPS对植物毒素冠菌素温度响应合成的调控
J Mol Microbiol Biotechnol. 2002 May;4(3):191-6.
8
Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis.在灵菌红素和绿脓菌素生物合成过程中L-脯氨酸向吡咯-2-羧基-S-PCP的转化。
Chem Biol. 2002 Feb;9(2):171-84. doi: 10.1016/s1074-5521(02)00100-x.
9
Determination by enantioselective synthesis of the absolute configuration of CPE, a potential intermediate in coronatine biosynthesis.
Org Lett. 2001 Sep 20;3(19):3045-7. doi: 10.1021/ol0165037.
10
4'-phosphopantetheine transfer in primary and secondary metabolism of Bacillus subtilis.枯草芽孢杆菌初级和次级代谢中的4'-磷酸泛酰巯基乙胺转移
J Biol Chem. 2001 Oct 5;276(40):37289-98. doi: 10.1074/jbc.M103556200. Epub 2001 Aug 6.
Zotero 插件