大肠杆菌丙二酰辅酶A-酰基载体蛋白转酰基酶编码基因fabD的克隆与核苷酸序列分析
Cloning and nucleotide sequence of the fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli.
作者信息
Magnuson K, Oh W, Larson T J, Cronan J E
机构信息
Department of Microbiology, University of Illinois, Urbana 61801.
出版信息
FEBS Lett. 1992 Mar 16;299(3):262-6. doi: 10.1016/0014-5793(92)80128-4.
PMID:1339356
Abstract
We report the cloning and nucleotide sequence of the gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli. Malonyl transacylase has been overexpressed 155-fold compared to a wild-type strain. Overexpression of this enzyme alters the fatty acid composition of a wild-type E. coli strain; increased amounts of cis-vaccenate are incorporated into the membrane phospholipids.
摘要
我们报道了大肠杆菌丙二酸单酰辅酶A-酰基载体蛋白转酰基酶编码基因的克隆及核苷酸序列。与野生型菌株相比，丙二酸单酰转酰基酶的表达量提高了155倍。该酶的过表达改变了野生型大肠杆菌菌株的脂肪酸组成；顺式- vaccenate的含量增加并掺入到膜磷脂中。
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