Sharma V D, Singh S P, Taku A
Department of Microbiology & Public Health, College of Veterinary Sciences, G.B. Pant University of Agriculture & Technology, Pantnagar, India.
Indian J Exp Biol. 1992 Jan;30(1):23-5.
Isolation of an enterotoxic factor from cell-free-culture-supernatant of S. stanley was achieved to homogeneity using salt precipitation, dialysis and molecular seive chromatography through Sephadex G-100 and G-200 columns. The purified enterotoxic factor yielded a single protein band on polyacrylamide gel electrophoresis, induced antibodies in the rabbit and showed single band on agar gel precipitation. It induced fluid accumulation in the rabbit ligated ileal loop (RLIL) and was neutralized by the homologous antiserum. Antigenically it was not related to cholera toxin but with enterotoxin of other Salmonella serotypes. It also exerted dermatotoxic effect in the rabbit skin causing marked central necrosis with peripheral erythema.
从斯坦利沙门氏菌无细胞培养上清液中分离出一种肠毒素因子，通过盐沉淀、透析以及经Sephadex G - 100和G - 200柱的分子筛色谱法，使其达到了均一性。纯化后的肠毒素因子在聚丙烯酰胺凝胶电泳上呈现单一蛋白条带，能在兔体内诱导产生抗体，在琼脂凝胶沉淀中也显示为单一沉淀带。它能在兔结扎回肠袢（RLIL）中诱导液体蓄积，且能被同源抗血清中和。在抗原性方面，它与霍乱毒素无关，而是与其他沙门氏菌血清型的肠毒素有关。它在兔皮肤中还发挥皮肤毒性作用，导致明显的中央坏死并伴有外周红斑。
