Harne S D, Sharma V D, Rahman H
Department of Microbiology and Public Health, College of Veterinary Sciences, G B Pant University of Agriculture and Technology, Pantnagar, India.
Indian J Exp Biol. 1990 Dec;28(12):1141-4.
Paw oedema test (POT) was standardized with modifications for the detection of Salmonella enterotoxin. Instead of measuring the weight of the inoculated paws after amputating the limbs at 48 hr post-inoculation, percent relative thickness of the order of 121 +/- 3.8% at 24-48 hr was found to be a better index. This test yielded parallel results to rabbit ligated ileal loop (RLIL) technique. The test was positive with enterotoxic crude cell lysates (CL) and cell free-culture-supernatants (CFCS) of S. newport and S. typhimurium, partially purified and purified enterotoxin of S. newport and purified cholera toxin. The test was found to be specific in that non-enterotoxic CFCS did not cause significant increase in the thickness. Minimum detection level of purified S. newport enterotoxin was estimated to be as low as 20 micrograms. Thus, the modified POT was considered to be an effective and economical bioassay model for the detection of Salmonella enterotoxin.
爪水肿试验（POT）经改良后标准化用于检测沙门氏菌肠毒素。接种后48小时截肢测量接种爪的重量，改为在24 - 48小时测量相对厚度百分比，发现121±3.8%左右是更好的指标。该试验结果与兔结扎回肠袢（RLIL）技术平行。新港沙门氏菌和鼠伤寒沙门氏菌的肠毒素粗细胞裂解物（CL）和无细胞培养上清液（CFCS）、新港沙门氏菌部分纯化和纯化的肠毒素以及纯化的霍乱毒素试验呈阳性。该试验具有特异性，因为非肠毒素CFCS不会导致厚度显著增加。纯化的新港沙门氏菌肠毒素的最低检测水平估计低至20微克。因此，改良的POT被认为是检测沙门氏菌肠毒素的一种有效且经济的生物测定模型。
