用于检测沙门氏菌肠毒素的酶联免疫吸附测定法（ELISA）和GM1-ELISA的评估。

Evaluation of ELISA and GM1-ELISA for detection of Salmonella enterotoxin.

作者信息

Rahman H, Singh V B, Sharma V D, Singh S P

机构信息

Department of Microbiology & Public Health, G.B. Pant, University of Agriculture and Technology, Pantnagar, India.

出版信息

Indian J Exp Biol. 1991 Oct;29(10):982-4.

PMID:1814840

Abstract

The ELISA and GM1-ELISA, by using antiserum to purified Salmonella enterotoxin (SE), were standardized and carried out to screen salmonellae isolated from foods of animal origin for enterotoxigenicity. Of the 101 strains of Salmonella belonging to 15 different serogroups tested, 76 (75.24%) strains from 13 serogroups were found enterotoxigenic. ELISA correlated well with rabbit ligated ileal loop (RLIL) test for the detection of enterotoxin producing salmonellae with 24 test strains. ELISA also yielded positive reaction with 7 of 13 RLIL negative strains. GM1-ELISA could not be carried out as none of the 101 cell free culture supernatants (CFCS) were able to bind with GM1-ganglioside. ELISA and GM1-ELISA were also standardized with antiserum to cholera toxin for the detection of salmonellae producing cholera related enterotoxin. None of the 101 strains was found to produce cholera related enterotoxin. ELISA could detect as low as 15 ng/100 microliters of purified SE and 10 ng/100 microliters of cholera toxin when tested with their homologous antisera.

摘要

利用抗纯化沙门氏菌肠毒素（SE）的抗血清，对酶联免疫吸附测定（ELISA）和GM1-ELISA进行了标准化，并用于筛选从动物性食品中分离出的沙门氏菌的产肠毒素能力。在测试的属于15个不同血清群的101株沙门氏菌中，发现来自13个血清群的76株（75.24%）具有产肠毒素能力。对于24株测试菌株，ELISA在检测产肠毒素沙门氏菌方面与兔结扎回肠袢（RLIL）试验相关性良好。ELISA对13株RLIL试验阴性菌株中的7株也产生了阳性反应。由于101份无细胞培养上清液（CFCS）均不能与GM1-神经节苷脂结合，因此无法进行GM1-ELISA。还利用抗霍乱毒素抗血清对ELISA和GM1-ELISA进行了标准化，以检测产霍乱相关肠毒素的沙门氏菌。未发现101株菌株中产霍乱相关肠毒素的情况。当用同源抗血清进行检测时，ELISA能够检测低至15 ng/100微升的纯化SE和10 ng/100微升的霍乱毒素。