Parshad R K, Guraya S S
Department of Zoology, Punjab Agricultural University, Ludhiana, India.
Indian J Exp Biol. 1992 Jan;30(1):5-7.
Chemotactic activity was measured in the follicular fluid collected from normal and atretic Graafian follicles isolated from the rat ovaries. The atresia of Graafian follicles was induced by pentobarbitone injections for 3 days beginning the day of proestrous. The chemotactic activity, as measured by direct morphological evaluation of cellular locomotion of individual cells and Boyden leading front assay, was significantly higher in follicular fluid from atretic follicles and it showed a progressive increase from day 1 to day 3 of blockade of ovulation. In vitro exposure of blocked follicles to PMSG and hCG on day 1, 2 and 3 failed to alter the chemotactic response of leukocytes towards follicular fluid of atretic follicles. Increased chemotaxis in the follicular fluid after 24 hr of blockade of ovulation appears to form an important criterion to identify atretic follicles well in advance, before the morphological symptoms of degeneration become apparent and the incipient change once induced in follicles is not reversed by gonadotropins.
在从大鼠卵巢分离出的正常和闭锁格拉夫卵泡中收集的卵泡液中测量趋化活性。从动情前期开始，通过注射戊巴比妥3天来诱导格拉夫卵泡闭锁。通过对单个细胞的细胞运动进行直接形态学评估和Boyden前沿分析法测量的趋化活性，在闭锁卵泡的卵泡液中显著更高，并且从排卵阻断的第1天到第3天呈逐渐增加。在第1、2和3天将闭锁卵泡体外暴露于孕马血清促性腺激素（PMSG）和人绒毛膜促性腺激素（hCG）未能改变白细胞对闭锁卵泡卵泡液的趋化反应。排卵阻断24小时后卵泡液中趋化性增加似乎形成了一个重要标准，可在卵泡退化的形态学症状明显出现之前提前很好地识别闭锁卵泡，并且一旦卵泡中诱导的初始变化不会被促性腺激素逆转。
