Parshad R K
Department of Zoology, Punjab Agricultural University, Ludhiana, India.
Indian J Exp Biol. 1994 Feb;32(2):86-8.
Surface membrane changes in the granulosa cells from follicles at different times following a single dose of PMSG have been investigated after the incubation of cells in media with and without hCG by spectrophotometric measure of concanavalin A-induced cellular agglutination rate. Agglutination rate and final level of agglutination of cells do not change from 24 to 48 hr but significantly rise at 72, 96 and 120 hr after PMSG administration. Incubation of cells in presence of hCG for 20 min decrease the agglutination level of cells at 24, 48 and 72 hr and no significant change was observed at 96 and 120 hr. The results, thus suggest that atretic changes when induced in the surface membrane of granulosa cells (after 72 hr of PMSG administration) cannot be reversed with hCG.
通过分光光度法测定伴刀豆球蛋白A诱导的细胞凝集率，研究了在给予单剂量孕马血清促性腺激素（PMSG）后不同时间卵泡颗粒细胞的表面膜变化。在含有和不含人绒毛膜促性腺激素（hCG）的培养基中培养细胞后发现，细胞的凝集率和最终凝集水平在PMSG给药后24至48小时内没有变化，但在72、96和120小时时显著升高。在hCG存在的情况下将细胞孵育20分钟，可降低24、48和72小时时细胞的凝集水平，而在96和120小时时未观察到显著变化。因此，结果表明，颗粒细胞表面膜诱导的闭锁变化（PMSG给药72小时后）不能被hCG逆转。
