Basu S K, Chuttani K, Srinivasan M N, Singh S, George S
Institute of Nuclear Medicine and Allied Sciences, Lucknow Marg, Delhi, India.
Indian J Exp Biol. 1992 Jun;30(6):478-81.
Differential radioprotection between normal tissues and carcinoma was observed in C3H/J mice treated with a combination of 5-hydroxy L-tryptophan (5-HTP, 100 mg/kg) and 2-aminoethylisothiuronium bromide hydrobromide (AET, 20 mg/kg). Protection to normal tissues was judged by LD50(30) and by radiation induced damage to bone marrow(BM) using clonogenic ability of blood forming stem cells (10 day CFUs) as the criteria. Pretreatment with 5-HTP + AET combination 30 min before whole body gamma radiation (WBGR) enhanced the recoveries of the number of blood forming stem cells in BM of irradiated mice after 0, 7th and 10th day of irradiation. LD50(30) for C3H/J mice was 7.3 Gy and the dose modifying factor (DMF) of 5-HTP + AET combination was 1.76. On the contrary, pretreatment with this combination did not protect the mammary carcinoma transplanted in C3H/J mice, when exposed to 80 Gy soft X-rays.
在用5-羟基-L-色氨酸（5-HTP，100毫克/千克）和氢溴酸2-氨基乙基异硫脲（AET，20毫克/千克）联合处理的C3H/J小鼠中，观察到正常组织和癌组织之间存在差异辐射防护作用。通过LD50(30)以及以造血干细胞的克隆形成能力（10天CFU）为标准，利用辐射对骨髓（BM）造成的损伤来判断对正常组织的保护作用。在全身γ辐射（WBGR）前30分钟用5-HTP + AET联合预处理，可提高受照射小鼠在照射后第0、7和10天骨髓中造血干细胞数量的恢复情况。C3H/J小鼠的LD50(30)为7.3 Gy，5-HTP + AET联合的剂量修正因子（DMF）为1.76。相反，当暴露于80 Gy软X射线时，用该联合预处理并不能保护移植于C3H/J小鼠体内的乳腺癌。
