Selvakumar N, Kumar V, Acharyulu G S, Rehman F, Paramasivan C N, Prabhakar R
Tuberculosis Research Centre, Madras.
Indian J Med Res. 1992 May;95:101-4.
A total of 114 strains of Mycobacterium tuberculosis isolated from sputum samples of 114 patients of pulmonary tuberculosis in south India, were coded and tested for their in vitro susceptibility to tuberactinomycin (Tum) incorporated in Lowenstein-Jensen (LJ) medium. Of these strains, 95 (83.3%) and 15 (13.2%) were susceptible to Tum at 25 and 50 mg/l respectively. Only 4 (3.5%) strains were inhibited at 100 mg/l or more. Of the 37 drug sensitive strains, 2 (5.4%) were not susceptible to Tum at 25 mg/l compared to 17 (22.1%) of 77 strains-resistant to one or more of antituberculosis drugs (P less than 0.02). The drug susceptibility pattern of the strains revealed that there was no significant association of resistance between Tum and streptomycin or rifampicin or ethambutol or ethionamide or isoniazid. However, 15 (53.6%) of 28 kanamycin (K) resistant strains were not susceptible to Tum at 25 mg/l. This cross resistance between Tum and K was further studied in 24 and 15 K sensitive and resistant strains respectively, by correlating their proportion resistance at 16 mg/l and it was found to have a significant positive correlation (r = 0.55; P less than 0.01).
从印度南部114例肺结核患者痰液样本中分离出的总共114株结核分枝杆菌，被编码并检测其对添加在罗-琴（LJ）培养基中的结核放线菌素（Tum）的体外敏感性。在这些菌株中，分别有95株（83.3%）和15株（13.2%）在25mg/l和50mg/l时对Tum敏感。只有4株（3.5%）在100mg/l及以上浓度时受到抑制。在37株药物敏感菌株中，2株（5.4%）在25mg/l时对Tum不敏感，而在77株对一种或多种抗结核药物耐药的菌株中，有17株（22.1%）不敏感（P<0.02）。菌株的药敏模式显示，Tum与链霉素、利福平、乙胺丁醇、乙硫异烟胺或异烟肼之间的耐药性无显著关联。然而，28株对卡那霉素（K）耐药的菌株中有15株（53.6%）在25mg/l时对Tum不敏感。分别在24株K敏感菌株和15株K耐药菌株中，通过关联它们在16mg/l时的比例耐药性，进一步研究了Tum与K之间的这种交叉耐药性，发现两者具有显著的正相关（r = 0.55；P<0.01）。
