Inoue Maiko, Gao Zhensheng, Hirata Mariko, Fujimori Masahiro, Cai Hongwei
Japan Grassland Farming and Forage Seed Association, Forage Crop Research Institute, Tochigi, Japan.
Genome. 2004 Feb;47(1):57-65. doi: 10.1139/g03-097.
To construct a high-density molecular linkage map of Italian ryegrass (Lolium multiflorum Lam), we used a two-way pseudo-testcross F1 population consisting of 82 individuals to analyze three types of markers: restriction fragment length polymorphism markers, which we detected by using genomic probes from Italian ryegrass as well as heterologous anchor probes from other species belonging to the Poaceae family, amplified fragment length polymorphism markers, which we detected by using PstI/MseI primer combinations, and telomeric repeat associated sequence markers. Of the restriction fragment length polymorphism probes that we generated from a PstI genomic library, 74% (239 of 323) of randomly selected probes detected hybridization patterns consistent with single-copy or low-copy genetic locus status in the screening. The 385 (mostly restriction fragment length polymorphism) markers that we selected from the 1226 original markers were grouped into seven linkage groups. The maps cover 1244.4 cM, with an average of 3.7 cM between markers. This information will prove useful for gene targeting, quantitative trait loci mapping, and marker-assisted selection in Italian ryegrass.
为构建多花黑麦草（Lolium multiflorum Lam）的高密度分子连锁图谱，我们使用了一个由82个个体组成的双拟测交F1群体，来分析三种类型的标记：限制性片段长度多态性标记（我们通过使用多花黑麦草的基因组探针以及禾本科其他物种的异源锚定探针来检测）、扩增片段长度多态性标记（我们通过使用PstI/MseI引物组合来检测）和端粒重复序列相关序列标记。在我们从PstI基因组文库中产生的限制性片段长度多态性探针中，74%（323个中的239个）随机选择的探针在筛选中检测到与单拷贝或低拷贝基因座状态一致的杂交模式。我们从1226个原始标记中选择的385个标记（大多数是限制性片段长度多态性标记）被分为七个连锁群。这些图谱覆盖1244.4厘摩，标记之间的平均距离为3.7厘摩。这些信息将被证明对多花黑麦草的基因定位、数量性状位点作图和标记辅助选择有用。
