Matsushima Hiroyuki, Mukai Kouichiro, Yoshida Shinichiro, Obara Yoshitaka
Department of Ophthalmology, Dokkyo University School of Medicine, Tochigi, Japan.
Jpn J Ophthalmol. 2004 Mar-Apr;48(2):97-100. doi: 10.1007/s10384-003-0029-8.
To analyze the effect of calcium on human lens epithelial cells (LECs) in vitro
Human LECs were obtained from the anterior lens capsule during a cataract operation, and were cultured in minimum essential medium (MEM) containing 12% fetal bovine serum for a week at 37 degrees C, 5% CO(2). The LECs were then isolated with trypsin and placed in culture dishes. To analyze the effects of calcium, the LECs were incubated in MEM with calcium concentrations of 0, 2, 10, or 20 mM. After H&E staining for 72 h, the LECs were analyzed with the Scion imaging program.
The LECs proliferated rapidly and their shapes were uniform in 2-mM-calcium MEM. The LECs proliferated more slowly and had irregular shapes in MEM with lower and higher concentrations of calcium.
Calcium affects both the proliferation and the shape of human LECs in culture. Abnormal (hyper- or hypo-) calcium concentrations in the lens and aqueous humor may change the homeostasis of LECs, resulting in cataracts and secondary posterior capsular opacification.
分析钙对体外培养的人晶状体上皮细胞（LECs）的影响。
在白内障手术过程中从晶状体前囊膜获取人LECs，并在含12%胎牛血清的最低必需培养基（MEM）中于37℃、5% CO₂条件下培养一周。然后用胰蛋白酶分离LECs并置于培养皿中。为分析钙的影响，将LECs在钙浓度分别为0、2、10或20 mM的MEM中孵育。用苏木精-伊红（H&E）染色72小时后，使用Scion成像程序对LECs进行分析。
在含2 mM钙的MEM中，LECs增殖迅速且形态均匀。在钙浓度较低和较高的MEM中，LECs增殖较慢且形态不规则。
钙影响培养的人LECs的增殖和形态。晶状体和房水中异常（高或低）的钙浓度可能会改变LECs的内环境稳态，导致白内障和继发性后囊膜混浊。
