台盼蓝染色对白内障晶状体上皮细胞密度和活力的影响

Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract.

作者信息

Nanavaty Mayank A, Johar Kaid, Sivasankaran Meena A, Vasavada Abhay R, Praveen Mamidipudi R, Zetterström Charlotta

机构信息

Iladevi Cataract & IOL Research Centre, Raghudeep Eye Clinic, Ahmedabad, India.

出版信息

J Cataract Refract Surg. 2006 Sep;32(9):1483-8. doi: 10.1016/j.jcrs.2006.04.017.

DOI:10.1016/j.jcrs.2006.04.017

PMID:16931259

Abstract

PURPOSE

To assess the effect of anterior capsule staining with trypan blue 0.0125% on the density and viability of the lens epithelial cells (LECs).

SETTING

Iladevi Cataract and IOL Research Center, Ahmedabad, India.

METHODS

This randomized prospective study comprised 40 eyes having phacoemulsification for white mature cataract. Patients were randomized to have the anterior capsule stained with 0.1 mL trypan blue 0.0125% solution before the anterior capsulorhexis (Group 1, n = 20) or to have no anterior capsule staining before the anterior capsulorhexis (Group 2, n = 20). The density and viability of LECs were analyzed. The first 20 samples (10 from Group 1, 10 from Group 2) were stained with hematoxylin-eosin for cell density analysis using image-analysis software. The remaining 20 samples (10 from each group) were stained with a solution containing 0.5 microM calcein AM and 5 microM propidium iodide for 10 minutes at 37 degrees C for viability assay.

RESULTS

The mean cell density in Group 1 (with staining) was 3533.15 cells/mm(2) +/- 664.01 (SD). This was significantly lower than the mean of 4235.59 +/- 414.93 cells/mm(2) in Group 2 (no staining) (P = .01). The mean capsule area covered with LECs (80.75% +/- 8.04% versus 94.63% +/- 3.78%) and the mean live LECs in the covered area (51.69% +/- 11.54% versus 68.67% +/- 9.15%) were also significantly lower in Group 1 (P = .002).

CONCLUSION

Staining the anterior capsule with trypan blue affected the density and viability of LECs.

摘要

目的

评估0.0125%台盼蓝对晶状体上皮细胞（LECs）密度和活力的影响。

背景

印度艾哈迈达巴德伊拉德维白内障与人工晶状体研究中心。

方法

这项随机前瞻性研究纳入了40只因白色成熟白内障接受超声乳化手术的眼睛。患者被随机分为两组，一组在撕囊前用0.1 mL 0.0125%台盼蓝溶液对前囊膜进行染色（第1组，n = 20），另一组在撕囊前不进行前囊膜染色（第2组，n = 20）。分析LECs的密度和活力。前20个样本（第1组10个，第2组10个）用苏木精-伊红染色，使用图像分析软件进行细胞密度分析。其余20个样本（每组10个）用含有0.5微摩尔钙黄绿素乙酰甲酯和5微摩尔碘化丙啶的溶液在37℃下染色10分钟进行活力测定。

结果

第1组（染色组）的平均细胞密度为3533.15个细胞/mm²±664.01（标准差）。这显著低于第2组（未染色组）的平均值4235.59±414.93个细胞/mm²（P = 0.01）。第1组覆盖有LECs的平均囊膜面积（80.75%±8.04%对94.63%±3.78%）和覆盖区域内的平均活LECs（51.69%±11.54%对68.67%±9.15%）也显著低于第2组（P = 0.002）。